Clone

Target

IDP00677  

Clone Suffix

1

Sequence Comments

 

Experiment Start Date

Jun 06, 2008  

Experiment End Date

Jun 07, 2008  

Person

Jason Stam  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

failed  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

353  

Reference Sequence Used

MSRITQVHRI LEQKHLDAII ILSDYNRRYL SGFTGTSGAL IISKDKQYLI TDFRYIDQAT KQAPNYEIIN RKSTIIGEIK ELLHQENFEN VGFEGHHVSY DTYLELNKSR ISLISISNTV DKIRDVKDAD EIALIQKAAN IVDETYEYIL TVVKAGMTEK ELKAILESKM LELGADGPSF DTIVASGHRG ALPHGVASDK IIEKGDMITL DFGAYYNGYC SDITRTFAIG EPDPKLKEIY QIVLESQMKA INEIRPGMTG AEADAISRNY LESKGYGKEF GHSLGHGIGL EIHEGPMLAR TIQDKLQVNN CVTVEPGVYI EGLGGIRIED DILITENGCQ VFTKCTKDLI VLT  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGAGCAGAATAACACAAGTGC  

PCR Backward Primer

TTATCCACTTCCAATGTTATGTTAAAACTATAAGGTCTTTT  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Aug 08, 2008