Clone

Target

IDP04627  

Clone Suffix

1

Sequence Comments

 

Experiment Start Date

Nov 17, 2009  

Experiment End Date

Nov 30, 2009  

Person

Sarah Grimshaw  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

453  

Reference Sequence Used

MSDVMADRTP PHNIEAEQAV LGAILIDQDA LTSASELLVP DSFYRTKHQK IFEVMLGLSD KGEPIDLVMM TSAMADQGLL EEVGGVSYLA ELAEVVPTAA NVEYYARIIA EKALLRRLIR TATHIVSDGY EREDDVDGLL NEAEKKILEV SHQTNAKAFQ NIKDVLVDAY DKIELLHNQK GEVTGIPTGF TELDKMTAGF QRNDLIIVAA RPSVGKTAFS LNIAQNVATK TDENVAIFSL EMGADQLVMR MLCAEGNIDA QRLRTGSLTS DDWAKLTMAM GSLSNAGIYI DDTPGIKVNE IRAKCRRLKQ EQGLGMILID YLQLIQGSGK SGENRQQEVS EISRTLKGIA RELQVPVIAL SQLSRGVESR QDKRPMMSDI RESGSIEQDA DIVAFLYRED YYDRETENKN TIEIIIAKQR NGPVGSVELA FVKEFNKFVN LERRFEDGHA PPA  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGAGTGATGTAATGGCTGATC  

PCR Backward Primer

TTATCCACTTCCAATGTTATGCCGGCGGTGCATGC  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Dec 03, 2009