Clone

Target

IDP04687  

Clone Suffix

1 - The clone is available in BEIR but it is out of stock.

Sequence Comments

 

Experiment Start Date

Nov 17, 2009  

Experiment End Date

Nov 30, 2009  

Person

Sarah Grimshaw  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

242  

Reference Sequence Used

MSGFFITATD TEVGKTVVAG ALAGVFRELG YNIGVYKALQ SGHVASNPEG DAARLKVLSG VPIKEDEICP YSIEEPLAPR LAMKRAGRAV TLKDIIHHYN ERLKEFNSLF VEGAGGLAVP YTEDALVIDF AKELQLPLIV VARPTLGTVN HTVLTIAYAK AHGLTVAGVI LSGCKECEME RVQENKVMIE ELSGVPVLGL LPFFEGEFTK EEVLESAKEY IMISKLEEFI RNESTVAHTS SN  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

MSGFFITATD TEVGKTVVAG ALAGVFRELG YNIGVYKALQ SGHVASNPEG DAARLKVLSG VPIKEDEICP YSIEEPLAPR LAMKRAGRAV TLKDIIHHYN ERLKEFNSLF VEGAGGLAVP YTEDALVIDF AKELQLPLIV VARPTLGTVN HTVLTIAYAK AHGLTVAGVI LSGCKECEME RVQENKVMIE ELSGVPVLGL LPFFEGEFTK EEVLESAKEY IMISKLEEFI RNESTVAHTS SN  

PCR Forward Primer

TACTTCCAATCCAATGCGATGAGCGGTTTTTTTATAACAGC  

PCR Backward Primer

TTATCCACTTCCAATGTTAGTTTGAAGATGTGTGCGC  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Dec 03, 2009