Clone

Target

IDP00788  

Clone Suffix

2

Sequence Comments

 

Experiment Start Date

Jun 06, 2008  

Experiment End Date

Jun 07, 2008  

Person

Jason Stam  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG19c  

Sequence Start

1  

Sequence End

493  

Reference Sequence Used

MKKIFMMVHE LDVNKGGMTS SMFNRSKEFY DADIPADIVT FDYKGNYDEI IKALKKQGKM DRRTKMYNVF EYFKQISNNK HFKSNKLLYK HISERLKNTI EIEESKGISR YFDITTGTYI AYIRKSKSEK VIDFFKDNKR IERFSFIDNK VHMKETFNVD NKVCYQVFYD EKGYPYISRN INANNGAVGK TYVLVNKKEF KNNLALCVYY LEKLIKDSKD SIMICDGPGS FPKMFNTNHK NAQKYGVIHV NHHENFDDTG AFKKSEKYII ENANKINGVI VLTEAQRLDI LNQFDVENIF TISNFVKIHN APKHFQTEKI VGHISRMVPT KRIDLLIEVA ELVVKKDNAV KFHIYGEGSV KDKIAKMIED KNLERNVFLK GYTTTPQKCL EDFKLVVSTS QYEGQGLSMI EAMISKRPVV AFDIKYGPSD FIEDNKNGYL IENHNINDMA DKILQLVNND VLAAEFGSKA RENIIEKYST ESILEKWLNL FNS  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGAAAAAAATATTTATGATGGTAC  

PCR Backward Primer

TTATCCACTTCCAATGTTAGCTATTGAAAAGATTTAACCA  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Aug 08, 2008