Clone

Target

IDP00485  

Clone Suffix

3 - The clone is available in BEIR and it is in stock.

Sequence Comments

 

Experiment Start Date

Apr 15, 2010  

Experiment End Date

Apr 15, 2010  

Person

Jason Stam  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

427  

Reference Sequence Used

MSSDRIVITG VAINTPIGDT LDLVGNNLLS GKSAIGYWRN FDTSNIYSKI GGQIPEYDIE GKLHAFSNRI PDIVLSRAEK VFKQTTKGVQ LSVLMAMEAW LDAGLFEHII DPFDCGIICG GHNLSDKYLL DQQKIFSDEP EFIDAFSGLN SLDTDHSSSV AQVLNTKGFA YLVGGTCATG NIAIQTAINA IKVDKKQVVA VVCPMASFHP MMLHSLTILE AICYSQYQDT PTKASRPYDI NRSGFLPSEG GAVLIFEQAE HAKNRGAKIY AELLGASVNS DANHLGDPSH EGQVRVIQDV LKKCNISPEQ VDYVSAHATS TKLGDIVELK SIKRVFGSHA EKLIINAPKS LLGHTMWASG VVEAAIAIWQ MRQGEFHQSH NIETLDPAVE LNVCASGNVK KEVNIFLNNS FGMGGINSAS LFSRYHG  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

MSSDRIVITG VAINTPIGDT LDLVGNNLLS GKSAIGYWRN FDTSNIYSKI GGQIPEYDIE GKLHAFSNRI PDIVLSRAEK VFKQTTKGVQ LSVLMAMEAW LDAGLFEHII DPFDCGIICG GHNLSDKYLL DQQKIFSDEP EFIDAFSGLN SLDTDHSSSV AQVLNTKGFA YLVGGTCATG NIAIQTAINA IKVDKKQVVA VVCPMASFHP MMLHSLTILE AICYSQYQDT PTKASRPYDI NRSGFLPSEG GAVLIFEQAE HAKNRGAKIY AELLGASVNS DANHLGDPSH EGQVRVIQDV LKKCNISPEQ VDYVSAHATS TKLGDIVELK SIKRVFGSHA EKLIINAPKS LLGHTMWASG VVEAAIAIWQ MRQGEFHQSH NIETLDPAVE LNVCASGNVK KEVNIFLNNS FGMGGINSAS LFSRYHG  

PCR Forward Primer

TACTTCCAATCCAATGCGATGTCTTCTGATCGTATCGTTA  

PCR Backward Primer

TTATCCACTTCCAATGTTAACCATGATATCGACTAAATAA  

Shipped To Lab

UT  

Shipped Date

Apr 29, 2010  

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

May 03, 2010