Clone

Target
IDP01229  
Clone Suffix
3
Experiment Start Date
Apr 15, 2010  
Experiment End Date
Apr 15, 2010  
Person
Jason Stam  
Lab
J. Craig Venter Institute  
Protocol ID
CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  
Status
success  
Vector
pMCSG7  
Sequence Start
1  
Sequence End
188  
Reference Sequence Used
MEERNEQVVE EVKEAQVEEA VTPENSEETV EEKSEAALLQ EKVDELQAKL TETEGRTLRL QADFENYKRR VQMDKQAAEK YRAQSLVSDI LPALDNFERA MQVEATDEQT KSLLQGMEMV HRQLLEALNK EGVEVIEAVG KQFDPNEHQA IMQVEDSEFE SNAVVEEFQK GYKLKDRVIR PSMVKVNQ  
Reference Sequence Variance
MATCH  
PCR Forward Primer
TACTTCCAATCCAATGCGATGGAAGAGCGTAACGAACAA  
PCR Backward Primer
TTATCCACTTCCAATGTTATTGATTTACTTTTACCATTGAT  
Shipped To Lab
UT  
Shipped Date
Jun 19, 2012  
Secondary Shipped To Lab
UT  
Secondary Shipped Date
Jun 19, 2012  
Inserted
May 03, 2010  

Related Expressions

   
Experiment
Date Start		 Experiment
Date End		 Person Lab Protocol Status Expression
Organism		 Media
Type		 Induction
Reagent		 Experiment
Type
2012-09-14 Maite Garcia University of Calgary Expression_protocol_Toronto success E. coli 15N 2X M9 none production
2010-04-15 2010-04-19 Jason Stam J. Craig Venter Institute CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins success E. coli 2xYT 1mM IPTG assay