Clone

Target

IDP90951  

Clone Suffix

3

Sequence Comments

 

Experiment Start Date

Apr 02, 2010  

Experiment End Date

Apr 07, 2010  

Person

Hanna Tekleab  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

failed  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

684  

Reference Sequence Used

MPAQTSSQLK HWFAKITSHS PFFFAILNDQ HQYVMVNERY CDIAGLSSEE MVGMSDSQVL GEHFYRHLKP FYERAFNNEH IESELTLSEI DLETSLHFSL SPIMINDRVQ YLVFHAIDTS EKQILVRSLE ESESKYALLT TLLPDGLMMV ENDCIISANP SAARLLGFDD AQKLLGENLS RLFIDEKTKT VFSSQLASLL TEKPLVCLTG PRCGFERKIQ LHAGCTSLLG NQSQLILLQD ADEAPKQFSA TTQVDAHIDS LTGLYNRHGF TKRLEQCIQN ETPLVMLYLD IDNFKNINDS LGHHIGDKVI KEVAARLKRL LPQQAVLGHL GGDEFGLILP EPEHNRSAEM LADRIISLIN QPFDLHHFSK RLACSIGSVR YPGDGNDARV LLQNADTAMY EAKERGRNRL IKFNDQMNKE ARMRLWLEIE LQKALQQNGL EVWYQPKVNA RDFSINGAEA LVRWKHPVEG YISPGAFIPV AEKAGLIEHL GRVVMREVFA TVKRWKLQGI LPGRVAINIS PEQFGNPQLI DYLEKLLRTT GLDPNNITFE LTESVVMSDS EHTQQMLNAI KKLGFTLSID DFGTGYSSLA YLARFPIDEL KIDRAFISNI DTLPKQLTVI ENIINLGRSL NLTVVAEGVE TQQQATLLSN LNCHSIQGFH FYRPQPKHEV EELFAQNRRH RKSL  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGCCTGCTCAAACCTCATC  

PCR Backward Primer

TTATCCACTTCCAATGTTAGAGGGATTTGCGATGGC  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

May 03, 2010