Clone

Target

IDP00167  

Clone Suffix

5

Sequence Comments

 

Experiment Start Date

May 28, 2010  

Experiment End Date

May 28, 2010  

Person

Hanna Tekleab  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

failed  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

4545  

Reference Sequence Used

MGKPFWRSVE YFFTGNYSAD DGNNNIVAIG FGGQIHAYGG DDHVTVGSIG ATVYTGSGND TVVGGSAYLK VEDSTGHLIV KGAAGYADIN KSGDGNVSFA GAAGGVSIDH LGNHGDVSYG GAAAYNGITR KGLSGNVTFA GAGGYNALWH ETNQGNLSFT GAGAGNKLDR TWSNRYQGSH GDVTFDGAGA ANSISSRVET GNITFRGAGA DNHLVRKGKV GDITLQGAGA SNRIERTHQA EDVYTQTRGN IRFEGVGGYN SLYSDVAHGD IHFSGGGAYN TIIRKGSGND FAKEGMTNAK ADEIVLTKAV MSGSWIGQDH HVTAVKSASE PNTYLFAFAD STYTKINKVQ LRNDPQTGEL KYYSTAWYKE VNHLSNLANQ DISDNGGFTA VNINGAYTLS DLKVEHQQSV TVHAVEKSLT EYEWVTYANG AVIDAKEVSL SDAKMGGHAI YADGTKVDVK AVKSNRQPNT YIYAKVLGPY TKIVVVELAN DPETGALKYQ ARSWYKEGDH TANIANQDIS SATGYNPMGK GGYSLSDLHY SVNAVRSTSE TVADIEEYTD QTLFKPANDS GESSGDVRFN GAGGGNVIKS NVTRGNVHFN GGGIANVILH SSQFGNTEFN GGGAANVIVK SGEEGDLTFR GAGLANVLVH QSEQGKMDVY AGGAVNVLVR LGDGQYLAHL LAYGNISVQK GSGDSRVVML GGYNTHTQIG SGNGLWLAAG GFNVMTQVGK GDVAAVLAGG ANVLTKMGEG ELTSGMLGGA NVITHISNDD QLSNTTAVAL GGANILTKKG KGNTLAVMGG GANVLTHVGD GTTTGVMVGG ANILTKVGNG DTTGILLGVG NVLTHVGDGQ TLGVMGAAGN IFTKVGDGTS IAVMIGAGNI FTHVGEGNAW ALMGGLGNVF TKVGNGDALA LMVAEANVFT HIGDGMSVAL MLAKGNVATK VGNGTTLAAM VGNVNIFTHI GHGSTFAAMI GQANIMTKVG NDLTAALMVG KANIMTHVGD GTSLGLFAGE VNVMTKVGNG TTLAAMFGKA NIMTHVGDGL TGVLALGEAN IVTKLGDDFM GVVAAAKANV VTHVGDATTA AVLAGKGNIL TKVGEGTTVG LLISDVGNVM THVGDGTTIG IAKGKANLIT KVGDGLGVNV TWGQANVFTQ VGDGDRYNFA KGEANLITKV GDGQEVSVVQ GEANIITHVG NGDDYTGAWG KANVITKVGH GQNVVLAKGE ANIVTQVGDG DSFNALWSKG NIVTKVGDGM QVTAAKGQAN ITTTVGNGLN VTAAYGDANI NTKVGDGVSV NVAWGKYNIN TKVGDGLNVA VMKGKANANI HVGDGLNINA SYAQNNVAIK VGNGDFYSLA VASSNTSSNK LSALFDNIKQ TVLGVGGSQA INYLVQGDEA SSSGTHKGRG AIATPEITKL DGFQMDAIKE VSSDLGDSLT GSVTKVDTPD LNKMQHALNV DDSSVQAPNL IVNGDFELGE HGWQSTHGVE ASYAGSVYGV EGEGHGARVT ELDTYTNTSL YQDLANLAQG EVIAVSFDFA KRAGLSNNEG IEVLWNGEVV FSSSXDESAW QQKNLKLTAQ AGSNRIEFKG TGHNDGLGYI LDNVVATSES SQQANAIREH ATQNPAAQNA LSDKERAEAD RQRLEQEKQK QLDAVAGSQS QLESTDQQAL ENNGQAQRDA VKEESEAVTA ELAKLAQGLD VLDGQATHTG ESGDQWRNDF AGGLLDGVQS QLDDAKQLAN DKIAAAKQTL SDNNSKVKES VAKSEAGVAQ GEQNRAGVEQ DIADAQADAE KRKADALAKG KDAQQAESDA HHAVNNAQSR GDRDVQLAEN KANQAQADAQ GAKQNEGDRP DRQGVTGSGL SGNAHSVEGA GETDSHVNTD SQTNADGRFS EGLTEQEQEA LEGATNAVNR LQINAGIRAK NSVSSMTSMF SETNSKSIVV PTKVSPEPER QEVTRRDVRI SGVNLESLSA VQGSQPTGQL ASKSVPGFKS HFASTSIGIE NELSGLVVVL PKNSAQTFGY VHDSQGNPLF MLTKDMNQGG YSNPVGINDI QGVNNWQTHT IELVTYPSEI SDTAAVESRK EAMLWLAKEF TDHINQSNHQ SLPHLVSDDG RFTLVISNSK HLIAAGNGTS IDAQGKTIGM TPSGQQATMA ISAKEFGTSS SPEVRLLESA PWYQAGLRDE FLANAKNTTL DDPATAQNVY AYLTSVYSKT ADLAKEYGIY INDWDPASEG FSPNAQGLTD PKVKNAWSIL PRTKPVRMLE LLSAEDSRYV RQQIAEKLKG TYSESLAKNV FEYFQYGGEV AGHGINNATT GSVQQPEPAI LFEFRSVPSA LSDFVPKTAS TVKVDVKALD HFDSASRKAI ITEVNALVSG SEDFDAWYQE YRASKGQPPV KNPKSSASAN HKAEWLMTQH AEQWAKITAP YTDNHETLTS TKLASNDKEE LHALGETSNL ENNKQQENVA SIINTMLNDM LPFYALRTER NLLVQEGDEG FEVRAWPGTE DKSKTIILED PEDAAQHKAI ERFILANFDN FEQMPDELFL VDNKVISHHE GRTHVLAQKV DGAWQYNATV ELMSVTELLD AANVTGKIRG ESYQQVIDAL TDYHASITEH ADYEPESVEK LLNLRKKIEG YVLGHPDSGR VEAMNSLLNQ VNTRLDEVSL LSVAEQTIQA QNSFSRLYDQ LEAANLKESK HLYLDQNGDF VTKGKGNLAN IDLLGSREAV LEKVKLTVSN EYGQTVADTI FAGLSAKDLA KDGKGVDIAG LNKVHQAIEQ HLSPVSATLY IWKPSDHSAL GHAALQIGQG RTQLEGQAAA DFNQQNYVSW WPLGSKSSNI SNILNVATKD QPDLKLRWSD FSQPAHQNDT LEHDVASEEN DGFGLHDGDI KLKRFIEKLN AAKGIDASFK EASEGYASVL LGNPDMLETT SIPAHVFQPF VEQWNDTSYD MMDVAHRFAQ ELRLQAQRSD DPELLEKRIG NVIRQFAERA LEEIETFKAS QADQGRVFRI NLEGLDVAAM QAEWHRLSND PDARYQLLTK NCSSTVAKVL KAGGADKLIG HTWLPKFGVW TPTELFNFGQ ALQEAQLEIA AKKQSHQVTD VLDALSGNEK PKENVAIEND GTPPRDKESL SPLTRFLNNE LYGDKEARRK IGEITQTLLD HAVEKGESQK ITLQGEAGRL TGYYHQGTAP SEGETSSPSG KVVLFLHGSG SSAEEQASAI RNHYQKQGID MLAVNLRGYG ESDGGPSEKG LYQDARTMFN YLVNDKGIDP SNIIIHGYSM GGPIAADLAR YAAQNGQAVS GLLLDRPMPS MTKAITAHEV ANPAGIVGAI AKAVNGQFSV EKNLEGLPKE TSILLLTDNE GLGNEGEKLR TKLTASGYNV TGEQTFYGHE ASNRLMSQYA DQIVSGLSSS ASVDEDLDQQ GLDTTSTKDQ GISNKNDHLQ VVDSKEALAD GKILHNQNVN SWGPITVTPT TDGGETRFDG QIIVQMENDP VVAKAAANLA GKHAESSVVV QLDSDGNYRV VYGDPSKLDG KLRWQLVGHG RDHSETNNTR LSGYSADELA VKLAKFQQSF NQAENINNKP DHISIVGCSL VSDDKQKGFG HQFINAMDAN GLRVDVSVRS SELAVDEAGR KHTKDANGDW VQKAENNKVS LSWDAQGEVV AKDERIRNGI AEGDIDLSRI GVNNVDEPAR GAIGDNNDVF DAPEKRKPET EVIANSSSSN QFSYSGNIQV NVGEGEFTAV NWGTSNVGIK VGTGGFKSLA FGDNNVMVHI GDGESKHSVD IGGYQALEGA QMFLGNRNVS FNFGHSNDLI LMMDKSIPTP PLVNPFDGAA RISGVLQGIA TSGEGEDWLA AQEQQWTLSG AKKFVKDMSG LDQSSSVDYT TLVELDSQNE RDSRGLKHDA EATLNKQYNQ WLSGNGNSGT SQLSRADKLR QANEKLAFNF AVGGQGADIQ VTTGNWNFMF GDNIQSILDT NLGSLFGLMT QQFTATGQAK TTFTYTPQDL PRQLKNKLLG QLAGVGAETT LADIFGVDYT ASGQIVSRNG QAVDGVAILK EMLEVIGEFS GDQLQAFVDP AKLLDSLKAG IDMGADGIKS FAETHGLKEK APEEEKDNSS VSVNGANVNS AQGATVADGN TETAETQDRA FGFNSLNLPN LFATIFSQDK QKEMKSLVEN LKQNLTADLL NMKEKTFDFL RNSGHLQGDG DINISLGNYN FNWGGDGKDL GAYLGDNNNF WGGRGDDVFY ATGKSNIFTG GEGNDMGVLM GRENMMFGGD GNDTAVVAGR INHVFLGAGD DQSFVFGEGG EIDTGSGRDY VVTSGNFNRV DTGDDQDYSV TIGNNNQVEL GAGNDFANIF GNYNRINAGA GNDVVKLMGY HAVLNGGDGD DHLIATAISK FSQFNGGEGR DLMVLGGYQN TFKGGTDVDS FVVSGDVIDN LVEDIRSEDN IVFNGIDWQK LWFERSGYDX KLSILRDPSN DSDQSKFEHI GSVTFSDYFN GNRAQVVIGM SEKDLSGERE YTMLSDSAID ALVQAMSGFE PQAGDNGFID SLESKSQAAI SMAWSDVVHK KGLMV  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGGGAAAACCATTTTGGAGAA  

PCR Backward Primer

TTATCCACTTCCAATGTTACACCATCAATCCTTTTTTGT  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Jul 26, 2010