Clone

Target

IDP01139  

Clone Suffix

4

Sequence Comments

 

Experiment Start Date

Apr 15, 2010  

Experiment End Date

Apr 15, 2010  

Person

Jason Stam  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

353  

Reference Sequence Used

MEKIERLRSA FDEAGIDGIL LTNEHSRRYM ANFTGTAGVV LISKKRAQFI TDFRYVEQAS KQAVGYEIVQ HAGLIIDEVA KQVKELGIQK LGFEQDTLTY SSYSAHKEAI DAEFIPTSGL VEKLRLIKTD SEIKILKEAA QIADAAFEHI LSFIRPGVSE IEVSNELEFF MRKQGATSSS FDIIVASGLR SALPHGVASE KVIETGDFVT LDFGAYYKGY CSDITRTIAV GEPSDKLKEI YNIVLEAQLR GVNGIKAGLT GREADALTRD YITEKGYGEY FGHSTGHGIG LEIHEAPGLA FRSDTVLEPG MAVTVEPGIY IPGIGGVRIE DDIIVTSEGN EVITKSPKEL IIL  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGGAGAAAATCGAAAGATTAAG  

PCR Backward Primer

TTATCCACTTCCAATGTTACAAAATAATAAGTTCTTTTGGT  

Shipped To Lab

ANL  

Shipped Date

Apr 29, 2010  

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Nov 24, 2010