Clone

Target

IDP00541  

Clone Suffix

1

Sequence Comments

 

Experiment Start Date

Jun 06, 2008  

Experiment End Date

Jun 07, 2008  

Person

Jason Stam  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

316  

Reference Sequence Used

MYTKILGTGS YLPVQVRSNA DLEKMVDTSD EWIVTRTGIR ERRIAGLDET VATMGFQAAE KALEMAGIDK DDIGLIIVAT TSSSHAFPSS ACQVQRMLGI KDAASFDLAA ACAGFTYALS VADQYVKSGA VKHAIVIGSD VLSRALDPED RGTIILFGDG AGAVVLGASE QPGIMSTHLH ADGRYGELLA LPYPDRQQDQ PAYVTMAGNE VFKVAVTELA HIVDETLQAN NLDRTALDWL VPHQANLRII SATAKKLGMG MDKVVITLDR HGNTSAASVP SAFDEAVRDG RIQRGQLVLL EAFGGGFTWG SALVRF  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGTATACTAAGATTCTCGGTAC  

PCR Backward Primer

TTATCCACTTCCAATGTCAAAAACGAACCAGTGCAGA  

Shipped To Lab

UT  

Shipped Date

Jul 16, 2008  

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Aug 08, 2008