Clone

Target
IDP05021  
Clone Suffix
1 The clone is not available in BEIR.
Experiment Start Date
Nov 06, 2010  
Experiment End Date
Nov 08, 2010  
Person
Sarah Grimshaw  
Lab
J. Craig Venter Institute  
Protocol ID
CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  
Status
success  
Vector
pMCSG7  
Sequence Start
1  
Sequence End
189  
Reference Sequence Used
MNSMVRLGGT LLAISAIAAL ALGATNQVTA PVIEQRNIQA NNELRKAVLP EAKEFKEMKE DTYKGLGDDL IAEVYEGLDG SEVVGYTLKA KPSGYGGEIE VMVGISSEGQ VTGVDIGNMS ETAGLGAKAK DDAFKGQYKG KTAEPLEVAK GSTTADNQIL AISGATITST AVTTGVNAAI DVFNSALNK  
Reference Sequence Variance
MATCH  
Confirmed Protein Sequence
MNSMVRLGGT LLAISAIAAL ALGATNQVTA PVIEQRNIQA NNELRKAVLP EAKEFKEMKE DTYKGLGDDL IAEVYEGLDG SEVVGYTLKA KPSGYGGEIE VMVGISSEGQ VTGVDIGNMS ETAGLGAKAK DDAFKGQYKG KTAEPLEVAK GSTTADNQIL AISGATITST AVTTGVNAAI DVFNSALNK  
PCR Forward Primer
TACTTCCAATCCAATGCGATGAACAGTATGGTGAGATTAG  
PCR Backward Primer
TTATCCACTTCCAATGTTATTTATTAAGAGCACTATTGAAA  
Shipped To Lab
ANL  
Shipped Date
Mar 03, 2011  
Secondary Shipped To Lab
UT  
Secondary Shipped Date
Sep 19, 2011  
Inserted
Nov 24, 2010  

Related Expressions

       
Experiment
Date Start		 Experiment
Date End		 Person Lab Protocol Status Expression
Organism		 Media
Type		 Induction
Reagent		 Experiment
Type
2011-05-24 2011-05-26 Magdalena Makowska-Grzyska Argonne National Laboratory standard expression_ANL success E. coli M9 minimal + semet 0.5 mM IPTG production
2010-11-17 2010-11-17 Sarah Grimshaw J. Craig Venter Institute CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins success E. coli 2xYT 1mM IPTG assay
2011-04-19 2011-04-21 Magdalena Makowska-Grzyska Argonne National Laboratory standard expression_ANL failed E. coli M9 minimal + semet 0.5 mM IPTG production
2012-02-27 Maite Garcia University of Calgary Expression_protocol_Toronto success E. coli 15N 2X M9 none production