Clone

Target

IDP00505  

Clone Suffix

1 - The clone is available in BEIR but it is out of stock.

Sequence Comments

 

Experiment Start Date

Jun 06, 2008  

Experiment End Date

Jun 07, 2008  

Person

Jason Stam  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

377  

Reference Sequence Used

MFQLSTQDIH LAAQADNKNA AITQVAAALT QAGCVTAAYV DGMLAREQQT STYLGNGIAI PHGTTDTRDQ VLNTGVQVFQ FPQGIEWGAD QTAYIVIGIA ARSDEHLALL RQLTHVLSDD AVAARLAKTT SAEELRSLLM GEQKTAEFHF DTSLIALDVA ADNLITLQAL NAGRLQQIGA VDARFVSDVI TREPLNLGQG IWLSDSTEGN LVSAVTISRP ATAFEHKGEK VALLLTLSVA DDQPLTVLNY LSELLLAQKA DALLNADAAA LLALLTSEYV EQSKVLSAEF VIRNEHGLHA RPGTILVNTI KQFTSEITVT NLDGTGKPAN GRSLMKVVAL GVKKGNRLRF TASGEDAQTA LDTIGEVIAA GLGEGAA  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

MFQLSTQDIH LAAQADNKNA AITQVAAALT QAGCVTAAYV DGMLAREQQT STYLGNGIAI PHGTTDTRDQ VLNTGVQVFQ FPQGIEWGAD QTAYIVIGIA ARSDEHLALL RQLTHVLSDD AVAARLAKTT SAEELRSLLM GEQKTAEFHF DTSLIALDVA ADNLITLQAL NAGRLQQIGA VDARFVSDVI TREPLNLGQG IWLSDSTEGN LVSAVTISRP ATAFEHKGEK VALLLTLSVA DDQPLTVLNY LSELLLAQKA DALLNADAAA LLALLTSEYV EQSKVLSAEF VIRNEHGLHA RPGTILVNTI KQFTSEITVT NLDGTGKPAN GRSLMKVVAL GVKKGNRLRF TASGEDAQTA LDTIGEVIAA GLGEGAA  

PCR Forward Primer

TACTTCCAATCCAATGCGATGTTTCAGCTATCTACGCAAG  

PCR Backward Primer

TTATCCACTTCCAATGTCATGCTGCCCCCTCGCC  

Shipped To Lab

UT  

Shipped Date

Jun 30, 2008  

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Aug 08, 2008