Clone

Target
IDP05274  
Clone Suffix
1
Experiment Start Date
Nov 06, 2010  
Experiment End Date
Nov 08, 2010  
Person
Sarah Grimshaw  
Lab
J. Craig Venter Institute  
Protocol ID
CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  
Status
success  
Vector
pMCSG7  
Sequence Start
1  
Sequence End
133  
Reference Sequence Used
MEVFVDGASA GNPGPSGAGI VLKADGIYEQ FAIPLAVMTN HEAEFIAIKL GLEEALKKQA TLIRLYSDSK VAIEAIHKRH AKNPLFKPHL DAILEMADSL ELFFAEWRNV SQNKQADQLA RQAIKKQKQP GVK  
Reference Sequence Variance
MATCH  
PCR Forward Primer
TACTTCCAATCCAATGCGATGGAAGTTTTCGTTGATGGTG  
PCR Backward Primer
TTATCCACTTCCAATGTTATTTGACTCCAGGTTGCTTC  
Shipped To Lab
UT  
Shipped Date
Jun 19, 2012  
Secondary Shipped To Lab
UT  
Secondary Shipped Date
Jun 19, 2012  
Inserted
Nov 24, 2010  

Related Expressions

       
Experiment
Date Start		 Experiment
Date End		 Person Lab Protocol Status Expression
Organism		 Media
Type		 Induction
Reagent		 Experiment
Type
2010-11-17 2010-11-17 Sarah Grimshaw J. Craig Venter Institute CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins success E. coli 2xYT 1mM IPTG assay
2011-04-26 2011-04-26 Sarah Grimshaw J. Craig Venter Institute CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins failed E. coli 2xYT 1mM IPTG assay
2011-10-31 2011-10-31 Sarah Grimshaw J. Craig Venter Institute CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins success E. coli 2xYT 1mM IPTG assay
2012-10-26 Maite Garcia University of Calgary Expression_protocol_Toronto success E. coli 15N 2X M9 none production