Clone

Target

IDP05312  

Clone Suffix

1

Sequence Comments

 

Experiment Start Date

Nov 06, 2010  

Experiment End Date

Nov 08, 2010  

Person

Sarah Grimshaw  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

304  

Reference Sequence Used

MRKIVLKDIL FLVTTLSIIV ILLLVTNITA NSKIKSTSET YLKSDKNSVS QNGIQSNTKN TAFKTTILST GKSDCILVEI GNKVIMIDTG EDKNGKQIVD RLKEKGINTL DYLILTHLDK DHIGGVDSVL SSVKVKNIIQ ANYKKDSKQY DEYIDSLKKA DIEPVLLKDN MNIVINSAEI NIHPASKSKY ESSNDYSIIT NISYGAYKFL FAGDAEEERL SEFINGNTLK YDFVKMPHHG RYDKLTETFL ESISPQYAVI TCSEKKEPEE GVLKILERLN IKTFLTSNGE VVINSDGKTL SVNQ  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGAGGAAAATAGTATTAAAAGATA  

PCR Backward Primer

TTATCCACTTCCAATGTTATTGATTCACACTCAATGTCT  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Nov 24, 2010