Clone

Target

IDP05315  

Clone Suffix

1

Sequence Comments

 

Experiment Start Date

Nov 06, 2010  

Experiment End Date

Nov 08, 2010  

Person

Sarah Grimshaw  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

267  

Reference Sequence Used

MTKIFILAST LSLSFFSGLN IGNAATENFS PITNAIVQNN DYFVEGNSKD NNKPPGNGGS QDDSGSDGNG SDGSGSGDNG SGDNGSGGNG SGGNGSGENG SGGNGSGSSG SGDNGSGGNG SGGNGSGSSG SGENGSGGNG SGSSGSGENG SGGNGSGSSG SGENGSGGNG SGGNGSGGNS SGGSGSGGSG SGDNGSGGSG SQGGNRVKGD SSSQGGNGSQ GGNVKDNAKK QGDKLPNTAT HYPASILMGL STFLIGMLLF IRRKNAK  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGACGAAAATTTTCATTTTAGCA  

PCR Backward Primer

TTATCCACTTCCAATGTTATTTTGCGTTTTTACGGCGA  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Nov 24, 2010