Clone

Target
IDP05405  
Clone Suffix
1
Experiment Start Date
Oct 20, 2010  
Experiment End Date
Nov 08, 2010  
Person
Sarah Grimshaw  
Lab
J. Craig Venter Institute  
Protocol ID
CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  
Status
success  
Vector
pMCSG7  
Sequence Start
1  
Sequence End
179  
Reference Sequence Used
MGVKKLFGFL SLFVICIVLV ACGVEEKTEV QLLKEMPKAK TMTIDPSLSK MEATEMVHAA QRFYAFWDTG KEELLSQTVT KDFFDNTLPK GRSQGIEGLK FAAQNFRKVV PDIHCKIEDL LVVGDKVTAR LSFSGTHNGK KINFFAIDIL HVKDGKITED WHLEDNLTLK QQLGLINEE  
Reference Sequence Variance
MATCH  
PCR Forward Primer
TACTTCCAATCCAATGCGATGGGGGTAAAAAAGTTGTTTG  
PCR Backward Primer
TTATCCACTTCCAATGCTATTCCTCGTTTATTAACCCA  

Related Expressions

     
Experiment
Date Start		 Experiment
Date End		 Person Lab Protocol Status Expression
Organism		 Media
Type		 Induction
Reagent		 Experiment
Type
2010-11-17 2010-11-17 Sarah Grimshaw J. Craig Venter Institute CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins success E. coli 2xYT 1mM IPTG assay
2011-04-26 2011-04-26 Sarah Grimshaw J. Craig Venter Institute CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins failed E. coli 2xYT 1mM IPTG assay
2011-10-31 2011-10-31 Sarah Grimshaw J. Craig Venter Institute CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins failed E. coli 2xYT 1mM IPTG assay