Clone

Target
IDP05454  
Clone Suffix
1 The clone is not available in BEIR.
Experiment Start Date
Jun 28, 2011  
Experiment End Date
Jun 28, 2011  
Person
James Winsor  
Lab
Northwestern University  
Protocol ID
NU_native_expression  
Status
success  
Vector
pMCSG 7  
Sequence Start
1  
Sequence End
155  
Reference Sequence Used
 
Reference Sequence Variance
MATCH  
Confirmed Protein Sequence
MRTLLSVLLA LMLVPALTGC KAPAKEDTTS NKKTTEEAKN ETPADLKLNF NEFSLDADYQ DTKKDYEADY KNVAADKKME AKIEDHKADV NLTGDEAITK LSPLLQELKF DKGTPDQEVI DQVLNVFKLD KDYQKFELEV VFSDGTKKEY KREIK  
PCR Forward Primer
 
PCR Backward Primer
 
Shipped To Lab
NU  
Shipped Date
Mar 21, 2011  
Secondary Shipped To Lab
UT  
Secondary Shipped Date
Sep 19, 2011  
Inserted
Nov 24, 2010  

Related Expressions

     
Experiment
Date Start		 Experiment
Date End		 Person Lab Protocol Status Expression
Organism		 Media
Type		 Induction
Reagent		 Experiment
Type
2011-05-18 2011-05-18 James Winsor Northwestern University NU_SelMet_expression success E. coli SeMet IPTG production
2010-11-17 2010-11-17 Sarah Grimshaw J. Craig Venter Institute CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins success E. coli 2xYT 1mM IPTG assay
2012-02-22 Maite Garcia University of Calgary Expression_protocol_Toronto success E. coli 15N 2X M9 none production