Clone

Target

IDP00522  

Clone Suffix

1

Sequence Comments

 

Experiment Start Date

Jun 06, 2008  

Experiment End Date

Jun 07, 2008  

Person

Jason Stam  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

183  

Reference Sequence Used

MLLENGWIVG VKQVLSPHFD LRPEGEMPSL LVIHSISLPP SEFGGPYIDQ LFTGTLNADE HPYFASIVHL RVSAHCLIRR DGEIIQYVPF DKRAWHAGVS VFAGRERCND FSIGIELEGT DVLPFTPVQY QRLTEISAVL FAHYPITVER VAGHSEIAPG RKTDPGLAFD WFFYQQNLAK LLV  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGCTGCTGGAAAACGGCTG  

PCR Backward Primer

TTATCCACTTCCAATGTCATACAAGCAACTTAGCTAAAT  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Aug 08, 2008