Clone

Target
IDP00073  
Clone Suffix
110
Sequence Comments
IDP00073.10-182.P(182)..pMCSG7  
Experiment Start Date
Jun 01, 2008  
Experiment End Date
Jun 01, 2008  
Person
Minyi Gu  
Lab
Argonne National Laboratory  
Protocol ID
standard cloning_ANL  
Status
success  
Vector
pMCSG7  
Sequence Start
10  
Sequence End
182  
Reference Sequence Used
PAEPVVLGKM GSSYGIRGWL RVFSSTEDAE SIFDYQPWFI QKAGQWQQVQ LESWKHHNQD LIIKLKGVDD RDAANLLTNC EIVVDSSQLP ALEEGDYYWK DLMGCQVVTA EGYDLGKVID MMETGSNDVL VIKANLKDAF GIKERLVPFL DGQVIKKVDL ATRTIEVDWD PGF  
PCR Forward Primer
TACTTCCAATCCAATGCCCCCGCTGAACCGGTTGTACTG  
PCR Backward Primer
TTATCCACTTCCAATGTTAAAAACCAGGATCCCAGTCTACTTCGATA  
Shipped To Lab
ANL  
Shipped Date
Jun 15, 2011  
Inserted
Jul 29, 2011  

Related Expressions

   
Experiment
Date Start		 Experiment
Date End		 Person Lab Protocol Status Expression
Organism		 Media
Type		 Induction
Reagent		 Experiment
Type
2008-07-01 2008-07-03 Minyi Gu Argonne National Laboratory standard expression_ANL success E. coli M9 minimal 1 mM IPTG assay
2008-04-09 2008-04-14 Natalia Maltseva Argonne National Laboratory standard expression_ANL success E. coli M9 minimal + semet 0.5 mM IPTG production