Clone

Target

IDP00425  

Clone Suffix

1

Sequence Comments

 

Experiment Start Date

Jun 06, 2008  

Experiment End Date

Jun 07, 2008  

Person

Jason Stam  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

420  

Reference Sequence Used

MSFETISVIG LGYIGLPTAA AFASRKKKVI GVDVNAHAVE TINRGAIHIV EPDLDKVVKI AVEGGYLQAV TKPQAADAFL IAVPTPFKGD HEPDMIFVES AAKSIAPVLK KGDLVILEST SPVGATEQMA QWLAEARPDL SFPQQAGEAA DINIAYCPER VLPGQVMVEL IQNDRVIGGM TPKCSARASA LYKIFLEGEC VVTNSRTAEM CKLTENSFRD VNIAFANELS LICDEQGINV WELIRLANRH PRVNILQPGP GVGGHCIAVD PWFIVSQNPQ LARLIHTARL VNDGKPLWVV DRVKAAVADC LAASDKRASE VKIACFGLAF KPDIDDLRES PAVGVARLIA EWHVGETLVV EPNVEQLPKS LMGLVTLKDT ATALQQADVL VMLVDHKQFK AIKPEDIKQQ WIVDTKGVWR  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGAGTTTTGAAACTATTTCTGTT  

PCR Backward Primer

TTATCCACTTCCAATGTCAACGCCATACTCCTTTGG  

Shipped To Lab

ANL  

Shipped Date

Jun 30, 2008  

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Aug 08, 2008