Clone

Target

IDP00424  

Clone Suffix

1

Sequence Comments

 

Experiment Start Date

Jun 06, 2008  

Experiment End Date

Jun 07, 2008  

Person

Jason Stam  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

411  

Reference Sequence Used

MAELPAKRRF TRIDRLPPYV FNITSELKMA ARRRGEDIID FSMGNPDGPT PPHIVEKMCS VAQREDTHGY STSRGIPCLR RAISRWYADR YQVDIDPENE AIVTIGSKEG LAHLMLATLD HGDTVLVPNP SYPIHIYGAV IAGAQVRSVP LTEGIDFFGE LERAIRETIP KPKMMILGFP SNPTAQCVEL DFFERVVALA KQYDVLVVHD LAYADIVYDG WKAPSIMQVP GAKDIAVEFF TLSKSYNMAG WRIGFMVGNP ELVSALARIK SYHDYGTFTP LQVAAIAALE GDQQCVRDIA EQYRQRRNAL VRGLHEAGWM VDNPKASMYV WAKIPEPYAH LGSLEFAKRL LAEAKVCVSP GIGFGDYGDT HVRFALIENQ DRIRQAVRGI KSMFRADGLL KPGKSSPNEM A  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGGCTGAATTACCTGCTAAAC  

PCR Backward Primer

TTATCCACTTCCAATGTTAGGCCATTTCATTGGGGG  

Shipped To Lab

ANL  

Shipped Date

Jul 16, 2008  

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Aug 08, 2008