Center for Structural Genomics of Infectious Diseases

Purification

Target: IDP01178
Purification ID: A10008
Expression: M9 minimal (Apr 18, 2012)
Experiment Date Start: Jun 11, 2012
Experiment Date End: Jun 12, 2012
Person: Magdalena Makowska-Grzyska
Lab: Argonne National Laboratory
Protocol: standard purification_ANL
Status: success
Description: IMAC-I, SEC::SEC peak 1::concentration and buffer exchange (Amicon 3k or 10k)
Affinity Tag Status: uncleaved
SeMet Status: native
Covalent Modification
Final Buffer: 20 mM HEPES pH 8.0, 250 mM NaCl, 2 mM DTT
Final Concentration (mg/mL): 48
Final Volume (μL): 1400
Shipped To Lab Id: ANL
Shipped Date: Jun 12, 2012
Inserted: Jun 15, 2012

Related Crystallization Drops

Experiment Date	Person	Lab	Protocol	Status	Method	Screen	Screen Component	Plate Type
Jun 12, 2012	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Index Screen	89	CrystalQuick standard profile, LBR round bottom plate
Jun 12, 2012	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-1	47	CrystalQuick standard profile, LBR round bottom plate
Jun 12, 2012	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-1	54	CrystalQuick standard profile, LBR round bottom plate
Jun 12, 2012	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-1	59	CrystalQuick standard profile, LBR round bottom plate
Jun 12, 2012	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-4	18	CrystalQuick standard profile, LBR round bottom plate
Jun 12, 2012	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-4	76	CrystalQuick standard profile, LBR round bottom plate
Jun 12, 2012	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-4	77	CrystalQuick standard profile, LBR round bottom plate
Jun 12, 2012	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Index Screen	59	CrystalQuick standard profile, LBR round bottom plate
Jun 12, 2012	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Index Screen	63	CrystalQuick standard profile, LBR round bottom plate
Jun 12, 2012	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Index Screen	89	CrystalQuick standard profile, LBR round bottom plate
Jun 12, 2012	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-1	47	CrystalQuick standard profile, LBR round bottom plate
Jun 12, 2012	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-1	54	CrystalQuick standard profile, LBR round bottom plate
Jun 12, 2012	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-1	59	CrystalQuick standard profile, LBR round bottom plate
Jun 12, 2012	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-1	73	CrystalQuick standard profile, LBR round bottom plate
Jun 12, 2012	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Index Screen	59	CrystalQuick standard profile, LBR round bottom plate
Jun 12, 2012	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Index Screen	63	CrystalQuick standard profile, LBR round bottom plate
Jun 12, 2012	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-4	18	CrystalQuick standard profile, LBR round bottom plate
Jun 12, 2012	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-4	63	CrystalQuick standard profile, LBR round bottom plate
Jun 12, 2012	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-4	76	CrystalQuick standard profile, LBR round bottom plate
Jun 12, 2012	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-4	77	CrystalQuick standard profile, LBR round bottom plate
Jun 12, 2012	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-4	93	CrystalQuick standard profile, LBR round bottom plate
Jun 12, 2012	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-4	96	CrystalQuick standard profile, LBR round bottom plate
Jun 12, 2012	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Index Screen	89	CrystalQuick standard profile, LBR round bottom plate
Jun 12, 2012	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-1	47	CrystalQuick standard profile, LBR round bottom plate
Jun 12, 2012	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-1	54	CrystalQuick standard profile, LBR round bottom plate
Jun 12, 2012	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Index Screen	59	CrystalQuick standard profile, LBR round bottom plate
Jun 12, 2012	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Index Screen	63	CrystalQuick standard profile, LBR round bottom plate
Jun 12, 2012	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-1	59	CrystalQuick standard profile, LBR round bottom plate
Jun 12, 2012	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Index Screen	92	CrystalQuick standard profile, LBR round bottom plate
Jun 12, 2012	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-4	89	CrystalQuick standard profile, LBR round bottom plate
Jun 12, 2012	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-4	59	CrystalQuick standard profile, LBR round bottom plate
Jun 12, 2012	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-4	63	CrystalQuick standard profile, LBR round bottom plate
Jun 12, 2012	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-4	96	CrystalQuick standard profile, LBR round bottom plate