Center for Structural Genomics of Infectious Diseases

Purification

Target: IDP92622
Purification ID: A11552
Expression: M9 minimal (Aug 07, 2012)
Experiment Date Start: Oct 01, 2012
Experiment Date End: Oct 03, 2012
Person: Magdalena Makowska-Grzyska
Lab: Argonne National Laboratory
Protocol: standard purification_ANL
Status: success
Description: IMAC-I, IMAC-II::ÃKTAxpress IMAC-II program flow-through (20 mM imidazole)::concentration and buffer exchange (Amicon 3k or 10k)
Affinity Tag Status: cleaved
SeMet Status: native
Covalent Modification
Final Buffer: 20 mM HEPES pH 8.0, 250 mM NaCl, 2 mM DTT
Final Concentration (mg/mL): 6
Final Volume (μL): 450
Shipped To Lab Id: ANL
Shipped Date: Oct 03, 2012
Inserted: Oct 04, 2012

Related Crystallization Drops

Experiment Date	Person	Lab	Protocol	Status	Method	Screen	Screen Component	Plate Type
Oct 02, 2012	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	nothing	sitting drop	Index Screen	95	CrystalQuick standard profile, LBR round bottom plate
Oct 02, 2012	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	nothing	sitting drop	MCSG-4	78	CrystalQuick standard profile, LBR round bottom plate
Oct 02, 2012	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	nothing	sitting drop	Index Screen	94	CrystalQuick standard profile, LBR round bottom plate
Oct 02, 2012	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	nothing	sitting drop	MCSG-3	40	CrystalQuick standard profile, LBR round bottom plate
Oct 02, 2012	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	nothing	sitting drop	MCSG-4	18	CrystalQuick standard profile, LBR round bottom plate
Oct 02, 2012	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	nothing	sitting drop	Index Screen	22	CrystalQuick standard profile, LBR round bottom plate
Oct 02, 2012	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	nothing	sitting drop	MCSG-3	57	CrystalQuick standard profile, LBR round bottom plate
Oct 02, 2012	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	nothing	sitting drop	MCSG-4	94	CrystalQuick standard profile, LBR round bottom plate
Oct 02, 2012	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	nothing	sitting drop	MCSG-3	52	CrystalQuick standard profile, LBR round bottom plate
Oct 02, 2012	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-4	37	CrystalQuick standard profile, LBR round bottom plate
Oct 02, 2012	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Index Screen	62	CrystalQuick standard profile, LBR round bottom plate
Oct 02, 2012	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Index Screen	89	CrystalQuick standard profile, LBR round bottom plate
Oct 02, 2012	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-3	85	CrystalQuick standard profile, LBR round bottom plate