Center for Structural Genomics of Infectious Diseases

Purification

Target: IDP91774
Purification ID: T69663
Expression: LB (Nov 13, 2012)
Experiment Date Start: Nov 13, 2012
Experiment Date End
Person: Elena Evdokimova
Lab: University of Calgary
Protocol: Purification_protocol_Toronto
Status: success
Description
Affinity Tag Status: cut,cleaved protein eluted with binding buffer
SeMet Status: native
Covalent Modification
Final Buffer
Final Concentration (mg/mL): 51
Final Volume (μL): 250
Shipped To Lab Id
Shipped Date
Inserted: Dec 11, 2012

Related Crystallization Drops

Experiment Date	Person	Lab	Protocol	Status	Method	Drop Precipitant Contents	pH
Dec 13, 2012	Elena Evdokimova	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	0.1M NH4Sulfate, 0.1M Na Cacodilate pH6.5, 30% PEG8K	6.5
Dec 13, 2012	Elena Evdokimova	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	0.1M NH4Sulfate, 0.1M Na Cacodilate pH6.5, 30% PEG8K	6.5
Dec 13, 2012	Elena Evdokimova	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	0.1M NH4Sulfate, 0.1M Na Cacodilate pH5.8, 30% PEG8K	5.8
Dec 13, 2012	Elena Evdokimova	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	0.1M NH4Sulfate, 0.1M Na Cacodilate pH6.5, 30% PEG8K	6.5
Dec 13, 2012	Elena Evdokimova	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	0.1M NH4Sulfate, 0.1M Na Cacodilate pH6.5, 30% PEG8K	6.5
Dec 13, 2012	Elena Evdokimova	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	0.1M NH4Sulfate, 0.1M Na Cacodilate pH5.8, 30% PEG8K	5.8
Dec 13, 2012	Elena Evdokimova	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	0.05M NH4Sulfate, 0.1M NaCacodilate pH6.8, 32%PEG8K	6.8
Dec 13, 2012	Elena Evdokimova	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	0.05M NH4Sulfate, 0.1M NaCacodilate pH6.8, 32%PEG8K	6.8
Dec 13, 2012	Elena Evdokimova	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	0.05M NH4Sulfate, 0.1M NaCacodilate pH6.8, 32%PEG8K	6.8
Dec 13, 2012	Elena Evdokimova	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	0.1M NH4Sulfate, 0.1M NaCacodilate pH6.9, 30%PEG8K	6.9
Dec 13, 2012	Elena Evdokimova	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	0.05M NH4Sulfate, 0.1M NaCacodilate pH6.8, 32%PEG8K	6.8
Dec 13, 2012	Elena Evdokimova	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	0.05M NH4Sulfate, 0.1M NaCacodilate pH6.8, 32%PEG8K	6.8
Dec 13, 2012	Elena Evdokimova	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	0.1M NH4Sulfate, 0.1M Na Cacodilate pH6.5, 30% PEG8K	6.5
Dec 13, 2012	Elena Evdokimova	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	0.2M NH4Sulfate, 0.1M Na Cacodilate pH5.8, 30% PEG8K	5.8
Dec 13, 2012	Elena Evdokimova	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	0.1M NH4Sulfate, 0.1M Na Cacodilate pH6.5, 30% PEG8K	6.5