Purification

Target
IDP02521  
Purification ID
A14786  
Expression
M9 minimal (May 20, 2013)  
Experiment Date Start
Jun 12, 2013  
Experiment Date End
Jun 18, 2013  
Person
Min Zhou  
Lab
Argonne National Laboratory  
Protocol
standard purification_ANL  
Status
success  
Description
IMAC-I, SEC, IMAC-II::ÄKTAxpress IMAC-II program flow-through (20 mM imidazole)::concentration and buffer exchange (Amicon 3k or 10k)  
Affinity Tag Status
na  
SeMet Status
native  
Final Buffer
20 mM HEPES pH 8.0, 250 mM NaCl, 2 mM DTT  
Final Concentration (mg/mL)
12  
Final Volume (μL)
250  
Shipped To Lab Id
ANL  
Shipped Date
Jun 21, 2013  
Inserted
Aug 05, 2013