Purification

Target
IDP00440  
Purification ID
A15862  
Expression
M9 minimal (Sep 16, 2013)  
Experiment Date Start
Sep 26, 2013  
Experiment Date End
Oct 02, 2013  
Person
Min Zhou  
Lab
Argonne National Laboratory  
Protocol
standard purification_ANL  
Status
success  
Description
IMAC-I, SEC, IMAC-II::ÄKTAxpress IMAC-II program flow-through (20 mM imidazole)::concentration and buffer exchange (Amicon 3k or 10k)  
Affinity Tag Status
na  
SeMet Status
native  
Final Buffer
20 mM HEPES pH 8.0, 250 mM NaCl, 2 mM DTT  
Final Concentration (mg/mL)
118  
Final Volume (μL)
1000  
Shipped To Lab Id
ANL  
Shipped Date
Oct 02, 2013  
Inserted
Oct 17, 2013