Purification

Target
IDP01423  
Purification ID
A16252  
Expression
M9 minimal (Nov 04, 2013)  
Experiment Date Start
Nov 13, 2013  
Experiment Date End
Nov 19, 2013  
Person
Min Zhou  
Lab
Argonne National Laboratory  
Protocol
standard purification_ANL  
Status
success  
Description
IMAC-I, SEC, IMAC-II::ÄKTAxpress IMAC-II program flow-through (20 mM imidazole)::concentration and buffer exchange (Amicon 3k or 10k)  
Affinity Tag Status
cleavage unsuccessful  
SeMet Status
native  
Final Buffer
20 mM HEPES pH 8.0, 250 mM NaCl, 2 mM DTT  
Final Concentration (mg/mL)
20  
Final Volume (μL)
300  
Shipped To Lab Id
ANL  
Shipped Date
Nov 19, 2013  
Inserted
Dec 05, 2013