Center for Structural Genomics of Infectious Diseases

Experiment Date	Person	Lab	Protocol	Status	Method	Drop Precipitant Contents	Screen	Screen Component	pH
May 14, 2009	Elena Gordon	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	polyethylene glycol 400 30 %v/v Magnesium Chloride hexahydrate .01 M Potassium Chloride .1 M Tris .05 M	CGC-trapSh	12	8.5
May 14, 2009	Elena Gordon	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	polyethylene glycol 400 15 %v/v Magnesium Chloride hexahydrate .2 M Sodium HEPES .1 M	CGC-trapSh	1	7.5
May 14, 2009	Elena Gordon	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Sodium Chloride 3.2 M Bis-Tris Propane .1 M	CGC-trapSh	10	7
May 14, 2009	Elena Gordon	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	iso-Propanol 5 %v/v ammonium sulfate 2 M	CGC-trapSh	13
May 14, 2009	Elena Gordon	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	ammonium sulfate 2 M potassium sodium tartrate tetrahydrate .2 M tri-Sodium Citrate dihydrate .1 M	CGC-trapSh	14	5.6
May 14, 2009	Elena Gordon	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Ammonium Sulfate 2.5 M Bis-Tris Propane .1 M	CGC-trapSh	20	7
May 14, 2009	Elena Gordon	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Glycerol Anhydrous 12 %v/v ammonium sulfate 1.5 M Tris .1 M	CGC-trapSh	22	8.5
May 14, 2009	Elena Gordon	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	polyethylene glycol 400 2 %v/v Ammonium Sulfate 2 M Sodium HEPES .1 M	CGC-trapSh	24	7.5
May 14, 2009	Elena Gordon	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Sodium Formate 3.5 M Sodium Acetate trihydrate .1 M	CGC-trapSh	26	4.6
May 14, 2009	Elena Gordon	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Sodium Nitrate 4 M Bis-Tris Propane .1 M	CGC-trapSh	27	7
May 14, 2009	Elena Gordon	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol 4000 20 % iso-Propanole 10 % tri-Sodium Citrate dihydrate .1 M	CGC-trapSh	36	5.6
May 14, 2009	Elena Gordon	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol Monomethyl Ether 2000 30 %w/v ammonium sulfate .2 M sodium acetate trihydrate .1 M	CGC-trapSh	48	4.6
May 14, 2009	Elena Gordon	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	PEG 5 KMME 25 % Ammonium Sulfate .2 M tri-Sodium Citrate dihydrate	CGC-trapSh	53	5.6
May 14, 2009	Elena Gordon	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol Monomethyl Ether 5000 30 %w/v ammonium sulfate .2 M MES .1 M	CGC-trapSh	54	6.5
May 14, 2009	Elena Gordon	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol 3350 20 %w/v Ammonium dihydrogen Phosphate .2 M	CGC-trapSh	59
May 14, 2009	Elena Gordon	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	polyethylene glycol 8000 18 %w/v Calcium Acetate hydrate .2 M Sodium Cacodylate .1 M	CGC-trapSh	60	6.5
May 14, 2009	Elena Gordon	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Lithium Sulfate monohydrate 1 M Bis-Tris Propane .1 M	CGC-trapSh	8	7
May 14, 2009	Elena Gordon	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Sodium Chloride 3.2 M Sodium Acetate trihydrate .1 M	CGC-trapSh	9	4.6
May 14, 2009	Elena Gordon	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	PEG 5KMME 25 % Ammonium Sulfate .2 M Tris .1 M	CGC-trapSh	55	8.5
May 14, 2009	Elena Gordon	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol 3350 20 %w/v di-Ammonium hydrogen Citrate .2 M	CGC-trapSh	57
May 14, 2009	Elena Gordon	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol 3350 20 %w/v Ammonium Formate .2 M	CGC-trapSh	58
May 14, 2009	Elena Gordon	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	polyethylene glycol 4000 30 %w/v Lithium Sulfate monohydrate .2 M Tris .1 M	CGC-trapSh	73	8.5
May 14, 2009	Elena Gordon	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol 3350 25 %w/v Lithium Sulfate monohydrate .2 M HEPES .1 M	CGC-trapSh	74	7.5
May 14, 2009	Elena Gordon	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol 3350 20 %w/v tri-Lthium Citrate tetrahydrate .2 M	CGC-trapSh	75
May 14, 2009	Elena Gordon	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol 3350 25 %w/v Lithium Sulfate monohydrate .2 M Bis-Tris .1 M	CGC-trapSh	76	5.5
May 14, 2009	Elena Gordon	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol 3350 25 %w/v Ammonium Acetate .2 M Bis-Tris .1 M	CGC-trapSh	77	5.5
May 14, 2009	Elena Gordon	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	polyethylene glycol 4000 30 %w/v Ammonium Acetate .2 M tri-Sodium Citrate dihydrate .1 M	CGC-trapSh	78	5.6
May 14, 2009	Elena Gordon	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	polyethylene glycol 4000 30 %w/v Ammonium Acetate .2 M Sodium acetate trihydrate .1 M	CGC-trapSh	79	4.6
May 14, 2009	Elena Gordon	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol 8000 30 %w/v Sodium Acetate trihydrate .2 M Bis-Tris	CGC-trapSh	80	6.5
May 14, 2009	Elena Gordon	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol 3350 20 %w/v di-Sodium Tartrate dihydrate .2 M	CGC-trapSh	81
May 14, 2009	Elena Gordon	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol 3350 25 % di-Sodium Tartrate dihydrate .2 M Tris .1 M	CGC-trapSh	82	8.5
May 14, 2009	Elena Gordon	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol 3350 20 %w/v Sodium Thiocyanate .2 M	CGC-trapSh	87
May 14, 2009	Elena Gordon	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	polyethylene glycol 4000 30 %w/v Sodium Acetate trihydrate .2 M Tris .1 M	CGC-trapSh	88	8.5
May 14, 2009	Elena Gordon	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol 3350 20 %w/v Potassium Chloride .2 M	CGC-trapSh	89
May 14, 2009	Elena Gordon	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol 3350 20 %w/v Potassium dihydrogen Phosphate .2 M	CGC-trapSh	90
May 14, 2009	Elena Gordon	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	polyethylene glycol 8000 20 %w/v Potassium dihydrogen Phosphate .05 M	CGC-trapSh	91
May 14, 2009	Elena Gordon	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol 3350 20 %w/v Potassium Sulfate .2 M	CGC-trapSh	92
May 14, 2009	Elena Gordon	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol 6000 10 %w/v MPD 5 %v/v HEPES .1 M	CGC-trapSh	93	7.5
May 14, 2009	Elena Gordon	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	tri-Ammonium Citrate pH 7.0 1 M Bis-Tris Propane .1 M	CGC-trapSh	2	7
May 14, 2009	Elena Gordon	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Ammonium Sulfate 1.6 M Calcium Chloride dihydrate .05 M Bis-Tris	CGC-trapSh	21	6.5
May 14, 2009	Elena Gordon	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Ammonium Acetate 4 M Sodium Acetate trihydrate .1 M	CGC-trapSh	4	4.6
May 14, 2009	Elena Gordon	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Ammonium Chloride 1.5 M Sodium Acetate trihydrate .1 M	CGC-trapSh	5	4.6
May 14, 2009	Elena Gordon	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Ammonium dihydrogen Phosphate 2 M Tris .1 M	CGC-trapSh	6	8.5
May 14, 2009	Elena Gordon	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	di-Ammonium Tartrate 1.1 M Sodium Acetate trihydrate .1 M	CGC-trapSh	7	4.6
May 14, 2009	Elena Gordon	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Sodium Chloride 5 M	CGC-trapSh	11
May 14, 2009	Elena Gordon	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	polyethylene glycol MME 5000 10 %w/v Tacsimate pH 7.0 5 %w/v HEPES .1 M	CGC-trapSh	33	7
May 14, 2009	Elena Gordon	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	iso-Propanol 5 %v/v polyethylene glycol 4000 10 %w/v Sodium HEPES .1 M	CGC-trapSh	34	7.5
May 14, 2009	Elena Gordon	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Sodium Formate 4 M	CGC-trapSh	25
May 14, 2009	Elena Gordon	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	tri-Ammonium Citrate pH 7.0 2 M Bis-Tris Propane .1 M	CGC-trapSh	3	7
May 14, 2009	Elena Gordon	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	polyethylene glycol 400 28 %v/v Calcium chloride dihydrate .2 M Sodium HEPES .1 M	CGC-trapSh	31	7.5
May 14, 2009	Elena Gordon	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol Monomethyl Ether 2000 30 % Ammonium Acetate .2 M tri-Sodium Citrate dihydrate	CGC-trapSh	32	5.6
May 14, 2009	Elena Gordon	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	ammonium sulfate 1.6 M Sodium Chloride .1 M HEPES .1 M	CGC-trapSh	19	7.5
May 14, 2009	Elena Gordon	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Ammonium Sulfate 1.5 M Ammonium Sulfate 1.5 M Bis-Tris	CGC-trapSh	18	6.5
May 14, 2009	Elena Gordon	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol 3350 20 %w/v Calcium chloride dihydrate .2 M	CGC-trapSh	63
May 14, 2009	Elena Gordon	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Ammonium Sulfate 1.5 M Sodium Acetate trihydrate .1 M	CGC-trapSh	15	4.6
May 14, 2009	Elena Gordon	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Ammonium Sulfate 2.5 M Sodium Acetate trihydrate .1 M	CGC-trapSh	16	4.6
May 14, 2009	Elena Gordon	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Ammonium Sulfate 2 M Bis-Tris .1 M	CGC-trapSh	17	5.5
May 14, 2009	Elena Gordon	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Ammonium Sulfate 2 M PEG 2KMME 10 %	CGC-trapSh	23
May 14, 2009	Elena Gordon	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol 3350 25 %w/v Magnesium Chloride hexahydrate .2 M Sodium acetate trihydrate .1 M	CGC-trapSh	65	4.6
May 14, 2009	Elena Gordon	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol 3350 25 %w/v Magnesium Chloride hexahydrate .2 M Tris .1 M	CGC-trapSh	70	8.5
May 14, 2009	Elena Gordon	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol Monomethyl Ether 2000 30 % Tris	CGC-trapSh	44	8.5
May 14, 2009	Elena Gordon	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	polyethylene glycol MME 5000 20 %w/v Bis-Tris .1 M	CGC-trapSh	45	6.5
May 14, 2009	Elena Gordon	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol 8000 20 % Sodium Chloride .2 M Phosphate-Citrate	CGC-trapSh	46	4.2
May 14, 2009	Elena Gordon	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol 10,000 20 % MES .1 M	CGC-trapSh	47	6
May 14, 2009	Elena Gordon	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	PEG2KMME 30 % Ammonium Sulfate .2 M Sodium Cacodylate .1 M	CGC-trapSh	49	6.5
May 14, 2009	Elena Gordon	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol 3350 25 %w/v ammonium sulfate .2 M Bis-Tris .1 M	CGC-trapSh	50	5.5
May 14, 2009	Elena Gordon	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol 3350 25 %w/v ammonium sulfate .2 M HEPES .1 M	CGC-trapSh	51	7.5
May 14, 2009	Elena Gordon	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	polyethylene glycol 8000 9 %w/v Calcium Acetate hydrate .2 M Sodium Cacodylate .1 M	CGC-trapSh	61	6.5
May 14, 2009	Elena Gordon	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol 3350 25 %w/v Calcium chloride dihydrate .2 M Tris .1 M	CGC-trapSh	62	8.5
May 14, 2009	Elena Gordon	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol 3350 25 %w/v Magnesium Chloride hexahydrate .2 M Bis-Tris .1 M	CGC-trapSh	68	6.5
May 14, 2009	Elena Gordon	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	polyethylene glycol 4000 30 %w/v Magnesium Chloride hexahydrate .2 M Tris .1 M	CGC-trapSh	71	8.5
May 14, 2009	Elena Gordon	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Sodium/Potassium Phosphate 1 M Sodium/Potassium Phosphate	CGC-trapSh	28	6.9
May 14, 2009	Elena Gordon	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	polyethylene glycol 8000 30 %w/v Ammonium Sulfate .2 M Sodium Cacodylate .1 M	CGC-trapSh	56	6.5
May 14, 2009	Elena Gordon	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol 3350 25 %w/v Magnesium Chloride hexahydrate .2 M tri-Sodium Citrate dihydrate .1 M	CGC-trapSh	66	5.6
May 14, 2009	Elena Gordon	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol 3350 25 %w/v Citric Acid .1 M	CGC-trapSh	37	3.5
May 14, 2009	Elena Gordon	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	polyethylene glycol 4000 25 %w/v Ammonium Sulfate .2 M Sodium acetate trihydrate .1 M	CGC-trapSh	52	4.6
May 14, 2009	Elena Gordon	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol 3350 20 %w/v Sodium dihydrogen Phosphate monohydrate .2 M	CGC-trapSh	83
May 14, 2009	Elena Gordon	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	iso-Propanol 15 %v/v Magnesium Chloride hexahydrate .025 M Potassium Chloride .1 M Sodium Cacodylate .05 M	CGC-trapSh	35	6
May 14, 2009	Elena Gordon	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol 3350 25 %w/v Magnesium Chloride hexahydrate .2 M Bis-Tris .1 M	CGC-trapSh	67	5.5
May 14, 2009	Elena Gordon	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	tri-Sodium Citrate dihydrate .7 M Tris .1 M	CGC-trapSh	29	8.5
May 14, 2009	Elena Gordon	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	tri-Sodium Citrate dihydrate 1.4 M Sodium HEPES .1 M	CGC-trapSh	30	7.5
May 14, 2009	Elena Gordon	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol 3350 25 %w/v Sodium Chloride .2 M Bis-Tris .1 M	CGC-trapSh	40	5.5
May 14, 2009	Elena Gordon	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol 3350 25 %w/v Bis-Tris .1 M	CGC-trapSh	43	6.5
May 14, 2009	Elena Gordon	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol 3350 20 %w/v potassium sodium tartrate tetrahydrate .2 M	CGC-trapSh	84
May 14, 2009	Elena Gordon	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Jeffamine ED-2001 reagent pH 7.0 30 %v/v HEPES .1 M	CGC-trapSh	85	7
May 14, 2009	Elena Gordon	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol 3350 20 %w/v Sodium Sulfate decahydrate .2 M	CGC-trapSh	86
May 14, 2009	Elena Gordon	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol 3350 15 %w/v Succinic Acid pH 7.0 .1 M	CGC-trapSh	94
May 14, 2009	Elena Gordon	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	polyethylene glycol MME 2000 1 %w/v Succinic Acid pH 7.0 1 M HEPES .1 M	CGC-trapSh	95	7
May 14, 2009	Elena Gordon	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol 3350 20 %w/v Calcium Acetate hydrate .2 M	CGC-trapSh	64
May 14, 2009	Elena Gordon	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol 3350 20 %w/v Zinc Acetate dihydrate .05 M	CGC-trapSh	96
May 14, 2009	Elena Gordon	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol 3350 25 %w/v Magnesium Chloride hexahydrate .2 M HEPES .1 M	CGC-trapSh	69	7.5
May 14, 2009	Elena Gordon	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol 3350 20 %w/v Magnesium Formate .2 M	CGC-trapSh	72
May 14, 2009	Elena Gordon	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol 3350 25 %w/v sodium acetate trihydrate .1 M	CGC-trapSh	38	4.5
May 14, 2009	Elena Gordon	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol 3350 25 %w/v Sodium Chloride .2 M tri-Sodium Citrate dihydrate	CGC-trapSh	39	5.6
May 14, 2009	Elena Gordon	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol 3350 25 %w/v Sodium Chloride .2 M HEPES .1 M	CGC-trapSh	41	7.5
May 14, 2009	Elena Gordon	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol 3350 25 %w/v Tris .1 M	CGC-trapSh	42	8.5

May 14, 2009

Elena Gordon

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

polyethylene glycol 400 30 %v/v Magnesium Chloride hexahydrate .01 M Potassium Chloride .1 M Tris .05 M

CGC-trapSh

12

8.5

May 14, 2009

Elena Gordon

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

polyethylene glycol 400 15 %v/v Magnesium Chloride hexahydrate .2 M Sodium HEPES .1 M

CGC-trapSh

1

7.5

May 14, 2009

Elena Gordon

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Sodium Chloride 3.2 M Bis-Tris Propane .1 M

CGC-trapSh

10

7

May 14, 2009

Elena Gordon

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

iso-Propanol 5 %v/v ammonium sulfate 2 M

CGC-trapSh

13

May 14, 2009

Elena Gordon

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

ammonium sulfate 2 M potassium sodium tartrate tetrahydrate .2 M tri-Sodium Citrate dihydrate .1 M

CGC-trapSh

14

5.6

May 14, 2009

Elena Gordon

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Ammonium Sulfate 2.5 M Bis-Tris Propane .1 M

CGC-trapSh

20

7

May 14, 2009

Elena Gordon

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Glycerol Anhydrous 12 %v/v ammonium sulfate 1.5 M Tris .1 M

CGC-trapSh

22

8.5

May 14, 2009

Elena Gordon

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

polyethylene glycol 400 2 %v/v Ammonium Sulfate 2 M Sodium HEPES .1 M

CGC-trapSh

24

7.5

May 14, 2009

Elena Gordon

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Sodium Formate 3.5 M Sodium Acetate trihydrate .1 M

CGC-trapSh

26

4.6

May 14, 2009

Elena Gordon

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Sodium Nitrate 4 M Bis-Tris Propane .1 M

CGC-trapSh

27

7

May 14, 2009

Elena Gordon

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol 4000 20 % iso-Propanole 10 % tri-Sodium Citrate dihydrate .1 M

CGC-trapSh

36

5.6

May 14, 2009

Elena Gordon

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol Monomethyl Ether 2000 30 %w/v ammonium sulfate .2 M sodium acetate trihydrate .1 M

CGC-trapSh

48

4.6

May 14, 2009

Elena Gordon

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

PEG 5 KMME 25 % Ammonium Sulfate .2 M tri-Sodium Citrate dihydrate

CGC-trapSh

53

5.6

May 14, 2009

Elena Gordon

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol Monomethyl Ether 5000 30 %w/v ammonium sulfate .2 M MES .1 M

CGC-trapSh

54

6.5

May 14, 2009

Elena Gordon

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol 3350 20 %w/v Ammonium dihydrogen Phosphate .2 M

CGC-trapSh

59

May 14, 2009

Elena Gordon

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

polyethylene glycol 8000 18 %w/v Calcium Acetate hydrate .2 M Sodium Cacodylate .1 M

CGC-trapSh

60

6.5

May 14, 2009

Elena Gordon

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Lithium Sulfate monohydrate 1 M Bis-Tris Propane .1 M

CGC-trapSh

8

7

May 14, 2009

Elena Gordon

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Sodium Chloride 3.2 M Sodium Acetate trihydrate .1 M

CGC-trapSh

9

4.6

May 14, 2009

Elena Gordon

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

PEG 5KMME 25 % Ammonium Sulfate .2 M Tris .1 M

CGC-trapSh

55

8.5

May 14, 2009

Elena Gordon

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol 3350 20 %w/v di-Ammonium hydrogen Citrate .2 M

CGC-trapSh

57

May 14, 2009

Elena Gordon

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol 3350 20 %w/v Ammonium Formate .2 M

CGC-trapSh

58

May 14, 2009

Elena Gordon

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

polyethylene glycol 4000 30 %w/v Lithium Sulfate monohydrate .2 M Tris .1 M

CGC-trapSh

73

8.5

May 14, 2009

Elena Gordon

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol 3350 25 %w/v Lithium Sulfate monohydrate .2 M HEPES .1 M

CGC-trapSh

74

7.5

May 14, 2009

Elena Gordon

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol 3350 20 %w/v tri-Lthium Citrate tetrahydrate .2 M

CGC-trapSh

75

May 14, 2009

Elena Gordon

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol 3350 25 %w/v Lithium Sulfate monohydrate .2 M Bis-Tris .1 M

CGC-trapSh

76

5.5

May 14, 2009

Elena Gordon

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol 3350 25 %w/v Ammonium Acetate .2 M Bis-Tris .1 M

CGC-trapSh

77

5.5

May 14, 2009

Elena Gordon

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

polyethylene glycol 4000 30 %w/v Ammonium Acetate .2 M tri-Sodium Citrate dihydrate .1 M

CGC-trapSh

78

5.6

May 14, 2009

Elena Gordon

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

polyethylene glycol 4000 30 %w/v Ammonium Acetate .2 M Sodium acetate trihydrate .1 M

CGC-trapSh

79

4.6

May 14, 2009

Elena Gordon

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol 8000 30 %w/v Sodium Acetate trihydrate .2 M Bis-Tris

CGC-trapSh

80

6.5

May 14, 2009

Elena Gordon

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol 3350 20 %w/v di-Sodium Tartrate dihydrate .2 M

CGC-trapSh

81

May 14, 2009

Elena Gordon

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol 3350 25 % di-Sodium Tartrate dihydrate .2 M Tris .1 M

CGC-trapSh

82

8.5

May 14, 2009

Elena Gordon

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol 3350 20 %w/v Sodium Thiocyanate .2 M

CGC-trapSh

87

May 14, 2009

Elena Gordon

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

polyethylene glycol 4000 30 %w/v Sodium Acetate trihydrate .2 M Tris .1 M

CGC-trapSh

88

8.5

May 14, 2009

Elena Gordon

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol 3350 20 %w/v Potassium Chloride .2 M

CGC-trapSh

89

May 14, 2009

Elena Gordon

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol 3350 20 %w/v Potassium dihydrogen Phosphate .2 M

CGC-trapSh

90

May 14, 2009

Elena Gordon

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

polyethylene glycol 8000 20 %w/v Potassium dihydrogen Phosphate .05 M

CGC-trapSh

91

May 14, 2009

Elena Gordon

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol 3350 20 %w/v Potassium Sulfate .2 M

CGC-trapSh

92

May 14, 2009

Elena Gordon

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol 6000 10 %w/v MPD 5 %v/v HEPES .1 M

CGC-trapSh

93

7.5

May 14, 2009

Elena Gordon

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

tri-Ammonium Citrate pH 7.0 1 M Bis-Tris Propane .1 M

CGC-trapSh

2

7

May 14, 2009

Elena Gordon

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Ammonium Sulfate 1.6 M Calcium Chloride dihydrate .05 M Bis-Tris

CGC-trapSh

21

6.5

May 14, 2009

Elena Gordon

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Ammonium Acetate 4 M Sodium Acetate trihydrate .1 M

CGC-trapSh

4

4.6

May 14, 2009

Elena Gordon

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Ammonium Chloride 1.5 M Sodium Acetate trihydrate .1 M

CGC-trapSh

5

4.6

May 14, 2009

Elena Gordon

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Ammonium dihydrogen Phosphate 2 M Tris .1 M

CGC-trapSh

6

8.5

May 14, 2009

Elena Gordon

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

di-Ammonium Tartrate 1.1 M Sodium Acetate trihydrate .1 M

CGC-trapSh

7

4.6

May 14, 2009

Elena Gordon

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Sodium Chloride 5 M

CGC-trapSh

11

May 14, 2009

Elena Gordon

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

polyethylene glycol MME 5000 10 %w/v Tacsimate pH 7.0 5 %w/v HEPES .1 M

CGC-trapSh

33

7

May 14, 2009

Elena Gordon

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

iso-Propanol 5 %v/v polyethylene glycol 4000 10 %w/v Sodium HEPES .1 M

CGC-trapSh

34

7.5

May 14, 2009

Elena Gordon

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Sodium Formate 4 M

CGC-trapSh

25

May 14, 2009

Elena Gordon

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

tri-Ammonium Citrate pH 7.0 2 M Bis-Tris Propane .1 M

CGC-trapSh

3

7

May 14, 2009

Elena Gordon

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

polyethylene glycol 400 28 %v/v Calcium chloride dihydrate .2 M Sodium HEPES .1 M

CGC-trapSh

31

7.5

May 14, 2009

Elena Gordon

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol Monomethyl Ether 2000 30 % Ammonium Acetate .2 M tri-Sodium Citrate dihydrate

CGC-trapSh

32

5.6

May 14, 2009

Elena Gordon

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

ammonium sulfate 1.6 M Sodium Chloride .1 M HEPES .1 M

CGC-trapSh

19

7.5

May 14, 2009

Elena Gordon

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Ammonium Sulfate 1.5 M Ammonium Sulfate 1.5 M Bis-Tris

CGC-trapSh

18

6.5

May 14, 2009

Elena Gordon

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol 3350 20 %w/v Calcium chloride dihydrate .2 M

CGC-trapSh

63

May 14, 2009

Elena Gordon

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Ammonium Sulfate 1.5 M Sodium Acetate trihydrate .1 M

CGC-trapSh

15

4.6

May 14, 2009

Elena Gordon

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Ammonium Sulfate 2.5 M Sodium Acetate trihydrate .1 M

CGC-trapSh

16

4.6

May 14, 2009

Elena Gordon

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Ammonium Sulfate 2 M Bis-Tris .1 M

CGC-trapSh

17

5.5

May 14, 2009

Elena Gordon

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Ammonium Sulfate 2 M PEG 2KMME 10 %

CGC-trapSh

23

May 14, 2009

Elena Gordon

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol 3350 25 %w/v Magnesium Chloride hexahydrate .2 M Sodium acetate trihydrate .1 M

CGC-trapSh

65

4.6

May 14, 2009

Elena Gordon

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol 3350 25 %w/v Magnesium Chloride hexahydrate .2 M Tris .1 M

CGC-trapSh

70

8.5

May 14, 2009

Elena Gordon

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol Monomethyl Ether 2000 30 % Tris

CGC-trapSh

44

8.5

May 14, 2009

Elena Gordon

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

polyethylene glycol MME 5000 20 %w/v Bis-Tris .1 M

CGC-trapSh

45

6.5

May 14, 2009

Elena Gordon

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol 8000 20 % Sodium Chloride .2 M Phosphate-Citrate

CGC-trapSh

46

4.2

May 14, 2009

Elena Gordon

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol 10,000 20 % MES .1 M

CGC-trapSh

47

6

May 14, 2009

Elena Gordon

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

PEG2KMME 30 % Ammonium Sulfate .2 M Sodium Cacodylate .1 M

CGC-trapSh

49

6.5

May 14, 2009

Elena Gordon

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol 3350 25 %w/v ammonium sulfate .2 M Bis-Tris .1 M

CGC-trapSh

50

5.5

May 14, 2009

Elena Gordon

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol 3350 25 %w/v ammonium sulfate .2 M HEPES .1 M

CGC-trapSh

51

7.5

May 14, 2009

Elena Gordon

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

polyethylene glycol 8000 9 %w/v Calcium Acetate hydrate .2 M Sodium Cacodylate .1 M

CGC-trapSh

61

6.5

May 14, 2009

Elena Gordon

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol 3350 25 %w/v Calcium chloride dihydrate .2 M Tris .1 M

CGC-trapSh

62

8.5

May 14, 2009

Elena Gordon

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol 3350 25 %w/v Magnesium Chloride hexahydrate .2 M Bis-Tris .1 M

CGC-trapSh

68

6.5

May 14, 2009

Elena Gordon

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

polyethylene glycol 4000 30 %w/v Magnesium Chloride hexahydrate .2 M Tris .1 M

CGC-trapSh

71

8.5

May 14, 2009

Elena Gordon

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Sodium/Potassium Phosphate 1 M Sodium/Potassium Phosphate

CGC-trapSh

28

6.9

May 14, 2009

Elena Gordon

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

polyethylene glycol 8000 30 %w/v Ammonium Sulfate .2 M Sodium Cacodylate .1 M

CGC-trapSh

56

6.5

May 14, 2009

Elena Gordon

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol 3350 25 %w/v Magnesium Chloride hexahydrate .2 M tri-Sodium Citrate dihydrate .1 M

CGC-trapSh

66

5.6

May 14, 2009

Elena Gordon

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol 3350 25 %w/v Citric Acid .1 M

CGC-trapSh

37

3.5

May 14, 2009

Elena Gordon

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

polyethylene glycol 4000 25 %w/v Ammonium Sulfate .2 M Sodium acetate trihydrate .1 M

CGC-trapSh

52

4.6

May 14, 2009

Elena Gordon

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol 3350 20 %w/v Sodium dihydrogen Phosphate monohydrate .2 M

CGC-trapSh

83

May 14, 2009

Elena Gordon

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

iso-Propanol 15 %v/v Magnesium Chloride hexahydrate .025 M Potassium Chloride .1 M Sodium Cacodylate .05 M

CGC-trapSh

35

6

May 14, 2009

Elena Gordon

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol 3350 25 %w/v Magnesium Chloride hexahydrate .2 M Bis-Tris .1 M

CGC-trapSh

67

5.5

May 14, 2009

Elena Gordon

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

tri-Sodium Citrate dihydrate .7 M Tris .1 M

CGC-trapSh

29

8.5

May 14, 2009

Elena Gordon

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

tri-Sodium Citrate dihydrate 1.4 M Sodium HEPES .1 M

CGC-trapSh

30

7.5

May 14, 2009

Elena Gordon

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol 3350 25 %w/v Sodium Chloride .2 M Bis-Tris .1 M

CGC-trapSh

40

5.5

May 14, 2009

Elena Gordon

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol 3350 25 %w/v Bis-Tris .1 M

CGC-trapSh

43

6.5

May 14, 2009

Elena Gordon

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol 3350 20 %w/v potassium sodium tartrate tetrahydrate .2 M

CGC-trapSh

84

May 14, 2009

Elena Gordon

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Jeffamine ED-2001 reagent pH 7.0 30 %v/v HEPES .1 M

CGC-trapSh

85

7

May 14, 2009

Elena Gordon

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol 3350 20 %w/v Sodium Sulfate decahydrate .2 M

CGC-trapSh

86

May 14, 2009

Elena Gordon

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol 3350 15 %w/v Succinic Acid pH 7.0 .1 M

CGC-trapSh

94

May 14, 2009

Elena Gordon

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

polyethylene glycol MME 2000 1 %w/v Succinic Acid pH 7.0 1 M HEPES .1 M

CGC-trapSh

95

7

May 14, 2009

Elena Gordon

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol 3350 20 %w/v Calcium Acetate hydrate .2 M

CGC-trapSh

64

May 14, 2009

Elena Gordon

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol 3350 20 %w/v Zinc Acetate dihydrate .05 M

CGC-trapSh

96

May 14, 2009

Elena Gordon

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol 3350 25 %w/v Magnesium Chloride hexahydrate .2 M HEPES .1 M

CGC-trapSh

69

7.5

May 14, 2009

Elena Gordon

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol 3350 20 %w/v Magnesium Formate .2 M

CGC-trapSh

72

May 14, 2009

Elena Gordon

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol 3350 25 %w/v sodium acetate trihydrate .1 M

CGC-trapSh

38

4.5

May 14, 2009

Elena Gordon

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol 3350 25 %w/v Sodium Chloride .2 M tri-Sodium Citrate dihydrate

CGC-trapSh

39

5.6

May 14, 2009

Elena Gordon

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol 3350 25 %w/v Sodium Chloride .2 M HEPES .1 M

CGC-trapSh

41

7.5

May 14, 2009

Elena Gordon

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol 3350 25 %w/v Tris .1 M

CGC-trapSh

42

8.5

Purification

Related Crystallization Drops