Purification

Target
IDP00418  
Purification ID
A16563  
Expression
M9 minimal (Oct 07, 2013)  
Experiment Date Start
Jan 13, 2014  
Experiment Date End
Jan 14, 2014  
Person
Natalia Maltseva  
Lab
Argonne National Laboratory  
Protocol
standard purification_ANL  
Status
success  
Description
IMAC-I, IMAC-II::ÄKTAxpress using IMAC-I program::ÄKTAxpress IMAC-I program eluate (250 mM imidazole)  
Affinity Tag Status
cleaved  
SeMet Status
native  
Final Buffer
20 mM HEPES pH 8.0, 250 mM NaCl, 2 mM DTT  
Final Concentration (mg/mL)
8  
Final Volume (μL)
22000  
Shipped To Lab Id
ANL  
Shipped Date
Jan 15, 2014  
Inserted
Jan 22, 2014