Center for Structural Genomics of Infectious Diseases

Purification

Target: IDP02508
Purification ID: A19886
Expression: M9 minimal (Nov 23, 2015)
Experiment Date Start: Dec 02, 2015
Experiment Date End: Dec 08, 2015
Person: Min Zhou
Lab: Argonne National Laboratory
Protocol: standard purification_ANL
Status: success
Description: IMAC-I, SEC, IMAC-II::ÃKTAxpress IMAC-II program flow-through (20 mM imidazole)::concentration and buffer exchange (Amicon 3k or 10k)
Affinity Tag Status: na
SeMet Status: native
Covalent Modification
Final Buffer: 20 mM HEPES pH 8.0, 250 mM NaCl, 2 mM DTT
Final Concentration (mg/mL): 95
Final Volume (μL): 400
Shipped To Lab Id: ANL
Shipped Date: Dec 09, 2015
Inserted: Dec 12, 2015

Experiment Date	Person	Lab	Protocol	Status	Method	Screen	Screen Component	Plate Type
Feb 02, 2016	Min Zhou	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-1	23	CrystalQuick standard profile, LBR round bottom plate
Dec 10, 2015	Min Zhou	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-4	67	CrystalQuick standard profile, LBR round bottom plate
Dec 10, 2015	Min Zhou	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-1	57	CrystalQuick standard profile, LBR round bottom plate
Dec 10, 2015	Min Zhou	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-4	26	CrystalQuick standard profile, LBR round bottom plate
Dec 10, 2015	Min Zhou	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-4	9	CrystalQuick standard profile, LBR round bottom plate
Dec 10, 2015	Min Zhou	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-1	23	CrystalQuick standard profile, LBR round bottom plate