Purification

Target
IDP55753  
Purification ID
A20381  
Expression
M9 minimal (Nov 07, 2011)  
Experiment Date Start
Jul 28, 2011  
Experiment Date End
Aug 05, 2011  
Person
Gyorgy Babnigg  
Lab
Argonne National Laboratory  
Protocol
standard purification_ANL  
Status
success  
Description
IMAC-I, IMAC-II::ÄKTAxpress IMAC-II program flow-through (20 mM imidazole)::concentration and buffer exchange (Amicon 3k or 10k)  
Affinity Tag Status
cleaved  
SeMet Status
native  
Final Buffer
50 mM HEPES pH 8.0, 500 mM NaCl, 5 % Glycerol, 10 mM ß-mercaptoethanol, 250 mM Imidazole  
Final Concentration (mg/mL)
6  
Final Volume (μL)
23774  
Shipped To Lab Id
ANL  
Shipped Date
Aug 05, 2011  
Inserted
Jun 15, 2016