Purification

Target

IDP64152  

Purification ID

A20333  

Expression

M9 minimal (Nov 11, 2013)  

Experiment Date Start

Nov 13, 2013  

Experiment Date End

Nov 22, 2013  

Person

Gyorgy Babnigg  

Lab

Argonne National Laboratory  

Protocol

standard purification_ANL  

Status

success  

Description

IMAC-I, IMAC-II::ÄKTAxpress IMAC-II program flow-through (20 mM imidazole)::concentration and buffer exchange (Amicon 3k or 10k)  

Affinity Tag Status

cleaved  

SeMet Status

native  

Covalent Modification

 

Final Buffer

50 mM HEPES pH 8.0, 500 mM NaCl, 5 % Glycerol, 10 mM ß-mercaptoethanol, 250 mM Imidazole  

Final Concentration (mg/mL)

443  

Final Volume (μL)

800  

Shipped To Lab Id

ANL  

Shipped Date

Nov 22, 2013  

Inserted

Jun 15, 2016