Purification

Target
IDP63255  
Purification ID
A20597  
Expression
M9 minimal (May 21, 2016)  
Experiment Date Start
Jun 01, 2016  
Experiment Date End
Jun 03, 2016  
Person
Gyorgy Babnigg  
Lab
Argonne National Laboratory  
Protocol
standard purification_ANL  
Status
success  
Description
IMAC-I, IMAC-II::ÄKTAxpress using IMAC-I program::ÄKTAxpress IMAC-I program eluate (250 mM imidazole)  
Affinity Tag Status
cleaved  
SeMet Status
native  
Final Buffer
50 mM HEPES pH 8.0, 500 mM NaCl, 5 % Glycerol, 10 mM ß-mercaptoethanol, 250 mM Imidazole  
Final Concentration (mg/mL)
6  
Final Volume (μL)
18500  
Shipped To Lab Id
ANL  
Shipped Date
Jun 03, 2016  
Inserted
Jul 16, 2016