Purification

Target

IDP56691  

Purification ID

A20363  

Expression

M9 minimal (May 01, 2013)  

Experiment Date Start

Jun 01, 2016  

Experiment Date End

Jun 03, 2016  

Person

Gyorgy Babnigg  

Lab

Argonne National Laboratory  

Protocol

standard purification_ANL  

Status

success  

Description

IMAC-I, IMAC-II::ÄKTAxpress IMAC-II program flow-through (20 mM imidazole)::concentration and buffer exchange (Amicon 3k or 10k)  

Affinity Tag Status

cleaved  

SeMet Status

native  

Covalent Modification

 

Final Buffer

50 mM HEPES pH 8.0, 500 mM NaCl, 5 % Glycerol, 10 mM ß-mercaptoethanol, 250 mM Imidazole  

Final Concentration (mg/mL)

341  

Final Volume (μL)

1200  

Shipped To Lab Id

ANL  

Shipped Date

Jun 03, 2016  

Inserted

Jul 27, 2016