Purification

Target
IDP93879  
Purification ID
V1087  
Expression
LB (Feb 04, 2018)  
Experiment Date Start
Feb 13, 2018  
Experiment Date End
Feb 14, 2018  
Person
Joanna Lipowska  
Lab
University of Virginia  
Protocol
uva_expression_native_1  
Status
failed  
Description
PRECYPITATED AFTER Ni-NTA!!! Pellet after digestion was jelly-like. Try Tris next time!  
Affinity Tag Status
na  
SeMet Status
native  
Final Buffer
20 mM HEPES,500 mM NaCl, 10% GOL, 150 mM Imidazole  
Final Concentration (mg/mL)
0  
Final Volume (μL)
0  
Inserted
Feb 14, 2018