Purification

Target
IDP96983  
Purification ID
A22163  
Expression
M9 minimal + semet (Jun 07, 2017)  
Experiment Date Start
Jun 09, 2017  
Experiment Date End
Jun 11, 2017  
Person
Jerzy Osipiuk  
Lab
Argonne National Laboratory  
Protocol
standard purification_ANL  
Status
success  
Description
IMAC-I, IMAC-II::ÄKTAxpress using IMAC-II program::ÄKTAxpress IMAC-II program flow-through (20 mM imidazole)  
Affinity Tag Status
cleaved  
SeMet Status
semet  
Final Buffer
50 mM HEPES pH 8.0, 500 mM NaCl, 5 % Glycerol, 10 mM ß-mercaptoethanol, 250 mM Imidazole  
Final Concentration (mg/mL)
3  
Final Volume (μL)
11111  
Shipped To Lab Id
ANL  
Shipped Date
Jun 11, 2017  
Inserted
Mar 03, 2018