Purification

Target

IDP96086  

Purification ID

A22505  

Expression

LB + 40 mM K2HPO4 (Jul 04, 2018)  

Experiment Date Start

Jul 06, 2018  

Experiment Date End

Jul 08, 2018  

Person

Lucas Welk  

Lab

Argonne National Laboratory  

Protocol

standard purification_ANL  

Status

success  

Description

IMAC-I, IMAC-II::ÄKTAxpress IMAC-II program flow-through (20 mM imidazole)::concentration and buffer exchange (Amicon 3k or 10k)  

Affinity Tag Status

na  

SeMet Status

native  

Covalent Modification

 

Final Buffer

20 mM HEPES pH 8.0, 250 mM NaCl, 2 mM DTT  

Final Concentration (mg/mL)

12  

Final Volume (μL)

100  

Shipped To Lab Id

ANL  

Shipped Date

Jul 08, 2018  

Inserted

Aug 08, 2018