Center for Structural Genomics of Infectious Diseases

Purification

Target: IDP97218
Purification ID: A22743
Expression: LB + 40 mM K2HPO4 (Aug 28, 2018)
Experiment Date Start: Sep 11, 2018
Experiment Date End: Sep 12, 2018
Person: Natalia Maltseva
Lab: Argonne National Laboratory
Protocol: standard purification_ANL
Status: success
Description: IMAC-I, IMAC-II::ÃKTAxpress IMAC-II program flow-through (20 mM imidazole)::concentration and buffer exchange (Amicon 3k or 10k)
Affinity Tag Status: cleaved
SeMet Status: native
Covalent Modification
Final Buffer: 20 mM HEPES pH 8.0, 250 mM NaCl, 2 mM DTT
Final Concentration (mg/mL): 71
Final Volume (μL): 400
Shipped To Lab Id: ANL
Shipped Date: Sep 13, 2018
Inserted: Nov 01, 2018

Related Crystallization Drops

Experiment Date	Person	Lab	Protocol	Status	Method	Screen	Screen Component	Plate Type
Sep 18, 2018	Natalia Maltseva	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-4	67	CrystalQuick standard profile, LBR round bottom plate
Sep 18, 2018	Natalia Maltseva	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	PEG/Ion HT Screen	64	CrystalQuick standard profile, LBR round bottom plate
Sep 18, 2018	Natalia Maltseva	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	PEG/Ion HT Screen	58	CrystalQuick standard profile, LBR round bottom plate
Apr 19, 2018	Natalia Maltseva	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	PEG/Ion HT Screen	56	CrystalQuick standard profile, LBR round bottom plate
Sep 18, 2018	Natalia Maltseva	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	PEG/Ion HT Screen	58	CrystalQuick standard profile, LBR round bottom plate
Sep 18, 2018	Natalia Maltseva	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-1	77	CrystalQuick standard profile, LBR round bottom plate
Sep 18, 2018	Natalia Maltseva	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-4	77	CrystalQuick standard profile, LBR round bottom plate
Sep 12, 2018	Natalia Maltseva	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Index Screen	86	CrystalQuick standard profile, LBR round bottom plate
Sep 12, 2018	Natalia Maltseva	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Index Screen	94	CrystalQuick standard profile, LBR round bottom plate
Sep 18, 2018	Natalia Maltseva	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	PEG/Ion HT Screen	56	CrystalQuick standard profile, LBR round bottom plate
Sep 12, 2018	Natalia Maltseva	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	PEG/Ion HT Screen	56	CrystalQuick standard profile, LBR round bottom plate
Sep 18, 2018	Natalia Maltseva	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	PEG/Ion HT Screen	94	CrystalQuick standard profile, LBR round bottom plate
Sep 18, 2018	Natalia Maltseva	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	PEG/Ion HT Screen	56	CrystalQuick standard profile, LBR round bottom plate
Sep 12, 2018	Natalia Maltseva	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	PEG/Ion HT Screen	56	CrystalQuick standard profile, LBR round bottom plate