Purification

Target
IDP01178  
Purification ID
A22764  
Expression
LB (Dec 05, 2017)  
Experiment Date Start
Dec 11, 2017  
Experiment Date End
Dec 13, 2017  
Person
Natalia Maltseva  
Lab
Argonne National Laboratory  
Protocol
standard purification_ANL  
Status
success  
Description
IMAC-I, IMAC-II::ÄKTAxpress IMAC-II program flow-through (20 mM imidazole)::concentration and buffer exchange (Amicon 3k or 10k)  
Affinity Tag Status
na  
SeMet Status
native  
Final Buffer
20 mM HEPES pH 8.0, 250 mM NaCl, 2 mM DTT  
Final Concentration (mg/mL)
54  
Final Volume (μL)
1100  
Shipped To Lab Id
ANL  
Shipped Date
Dec 13, 2017  
Inserted
Nov 02, 2018