Center for Structural Genomics of Infectious Diseases

Experiment Date	Person	Lab	Protocol	Status	Method	Drop Precipitant Contents	pH
Jan 26, 2011	Olena Onopriyenko	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Sodium Chloride 5 M
Jan 26, 2011	Olena Onopriyenko	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	iso-Propanol 5 %v/v ammonium sulfate 2 M
Jan 26, 2011	Olena Onopriyenko	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Ammonium Sulfate 2 M Bis-Tris .1 M	5.5
Jan 26, 2011	Olena Onopriyenko	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	iso-Propanol 5 %v/v polyethylene glycol 4000 10 %w/v Sodium HEPES .1 M	7.5
Jan 26, 2011	Olena Onopriyenko	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol 3350 20 %w/v Ammonium Formate .2 M
Jan 26, 2011	Olena Onopriyenko	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	polyethylene glycol 8000 18 %w/v Calcium Acetate hydrate .2 M Sodium Cacodylate .1 M	6.5
Jan 26, 2011	Olena Onopriyenko	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol 8000 30 %w/v Sodium Acetate trihydrate .2 M Bis-Tris	6.5
Jan 26, 2011	Olena Onopriyenko	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol 3350 20 %w/v potassium sodium tartrate tetrahydrate .2 M
Jan 26, 2011	Olena Onopriyenko	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol 3350 15 %w/v Succinic Acid pH 7.0 .1 M
Jan 26, 2011	Olena Onopriyenko	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Sodium Formate 4 M
Jan 26, 2011	Olena Onopriyenko	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol 3350 20 %w/v Sodium Thiocyanate .2 M
Jan 26, 2011	Olena Onopriyenko	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Glycerol Anhydrous 12 %v/v ammonium sulfate 1.5 M Tris .1 M	8.5
Jan 26, 2011	Olena Onopriyenko	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol 3350 20 %w/v Sodium dihydrogen Phosphate monohydrate .2 M
Jan 26, 2011	Olena Onopriyenko	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	iso-Propanol 15 %v/v Magnesium Chloride hexahydrate .025 M Potassium Chloride .1 M Sodium Cacodylate .05 M	6
Jan 26, 2011	Olena Onopriyenko	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol 3350 25 %w/v Citric Acid .1 M	3.5
Jan 26, 2011	Olena Onopriyenko	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol 3350 25 %w/v sodium acetate trihydrate .1 M	4.5
Jan 26, 2011	Olena Onopriyenko	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol 3350 25 %w/v Sodium Chloride .2 M tri-Sodium Citrate dihydrate	5.6
Jan 26, 2011	Olena Onopriyenko	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol 3350 25 %w/v Sodium Chloride .2 M Bis-Tris .1 M	5.5
Jan 26, 2011	Olena Onopriyenko	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol 3350 25 %w/v Sodium Chloride .2 M HEPES .1 M	7.5
Jan 26, 2011	Olena Onopriyenko	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol 3350 25 %w/v Tris .1 M	8.5
Jan 26, 2011	Olena Onopriyenko	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol 3350 25 %w/v Bis-Tris .1 M	6.5
Jan 26, 2011	Olena Onopriyenko	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol Monomethyl Ether 2000 30 % Tris	8.5
Jan 26, 2011	Olena Onopriyenko	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	polyethylene glycol MME 5000 20 %w/v Bis-Tris .1 M	6.5
Jan 26, 2011	Olena Onopriyenko	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol 8000 20 % Sodium Chloride .2 M Phosphate-Citrate	4.2
Jan 26, 2011	Olena Onopriyenko	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol 10,000 20 % MES .1 M	6
Jan 26, 2011	Olena Onopriyenko	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol 3350 25 %w/v ammonium sulfate .2 M Bis-Tris .1 M	5.5
Jan 26, 2011	Olena Onopriyenko	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol 3350 25 %w/v ammonium sulfate .2 M HEPES .1 M	7.5
Jan 26, 2011	Olena Onopriyenko	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	polyethylene glycol 4000 25 %w/v Ammonium Sulfate .2 M Sodium acetate trihydrate .1 M	4.6
Jan 26, 2011	Olena Onopriyenko	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	PEG 5 KMME 25 % Ammonium Sulfate .2 M tri-Sodium Citrate dihydrate	5.6
Jan 26, 2011	Olena Onopriyenko	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol 3350 25 %w/v Calcium chloride dihydrate .2 M Tris .1 M	8.5
Jan 26, 2011	Olena Onopriyenko	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol 3350 20 %w/v Calcium chloride dihydrate .2 M
Jan 26, 2011	Olena Onopriyenko	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol 3350 20 %w/v Calcium Acetate hydrate .2 M
Jan 26, 2011	Olena Onopriyenko	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	polyethylene glycol 4000 30 %w/v Lithium Sulfate monohydrate .2 M Tris .1 M	8.5
Jan 26, 2011	Olena Onopriyenko	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol 3350 20 %w/v tri-Lthium Citrate tetrahydrate .2 M
Jan 26, 2011	Olena Onopriyenko	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol 6000 10 %w/v MPD 5 %v/v HEPES .1 M	7.5
Jan 26, 2011	Olena Onopriyenko	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	ammonium sulfate 2 M potassium sodium tartrate tetrahydrate .2 M tri-Sodium Citrate dihydrate .1 M	5.6
Jan 26, 2011	Olena Onopriyenko	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Ammonium Sulfate 2.5 M Sodium Acetate trihydrate .1 M	4.6
Jan 26, 2011	Olena Onopriyenko	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Ammonium Sulfate 1.5 M Ammonium Sulfate 1.5 M Bis-Tris	6.5
Jan 26, 2011	Olena Onopriyenko	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Ammonium Sulfate 2 M PEG 2KMME 10 %
Jan 26, 2011	Olena Onopriyenko	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	polyethylene glycol 400 2 %v/v Ammonium Sulfate 2 M Sodium HEPES .1 M	7.5
Jan 26, 2011	Olena Onopriyenko	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Sodium Formate 3.5 M Sodium Acetate trihydrate .1 M	4.6
Jan 26, 2011	Olena Onopriyenko	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Sodium Nitrate 4 M Bis-Tris Propane .1 M	7
Jan 26, 2011	Olena Onopriyenko	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Sodium/Potassium Phosphate 1 M Sodium/Potassium Phosphate	6.9
Jan 26, 2011	Olena Onopriyenko	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	tri-Ammonium Citrate pH 7.0 2 M Bis-Tris Propane .1 M	7
Jan 26, 2011	Olena Onopriyenko	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	tri-Sodium Citrate dihydrate 1.4 M Sodium HEPES .1 M	7.5
Jan 26, 2011	Olena Onopriyenko	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	polyethylene glycol 400 28 %v/v Calcium chloride dihydrate .2 M Sodium HEPES .1 M	7.5
Jan 26, 2011	Olena Onopriyenko	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol Monomethyl Ether 2000 30 % Ammonium Acetate .2 M tri-Sodium Citrate dihydrate	5.6
Jan 26, 2011	Olena Onopriyenko	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	polyethylene glycol MME 5000 10 %w/v Tacsimate pH 7.0 5 %w/v HEPES .1 M	7
Jan 26, 2011	Olena Onopriyenko	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Ammonium Acetate 4 M Sodium Acetate trihydrate .1 M	4.6
Jan 26, 2011	Olena Onopriyenko	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Ammonium Chloride 1.5 M Sodium Acetate trihydrate .1 M	4.6
Jan 26, 2011	Olena Onopriyenko	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol Monomethyl Ether 5000 30 %w/v ammonium sulfate .2 M MES .1 M	6.5
Jan 26, 2011	Olena Onopriyenko	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	PEG 5KMME 25 % Ammonium Sulfate .2 M Tris .1 M	8.5
Jan 26, 2011	Olena Onopriyenko	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	polyethylene glycol 8000 30 %w/v Ammonium Sulfate .2 M Sodium Cacodylate .1 M	6.5
Jan 26, 2011	Olena Onopriyenko	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol 3350 20 %w/v Ammonium dihydrogen Phosphate .2 M
Jan 26, 2011	Olena Onopriyenko	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Ammonium dihydrogen Phosphate 2 M Tris .1 M	8.5
Jan 26, 2011	Olena Onopriyenko	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	polyethylene glycol 8000 9 %w/v Calcium Acetate hydrate .2 M Sodium Cacodylate .1 M	6.5
Jan 26, 2011	Olena Onopriyenko	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol 3350 25 %w/v Magnesium Chloride hexahydrate .2 M Sodium acetate trihydrate .1 M	4.6
Jan 26, 2011	Olena Onopriyenko	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol 3350 25 %w/v Magnesium Chloride hexahydrate .2 M tri-Sodium Citrate dihydrate .1 M	5.6
Jan 26, 2011	Olena Onopriyenko	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol 3350 25 %w/v Magnesium Chloride hexahydrate .2 M Bis-Tris .1 M	5.5
Jan 26, 2011	Olena Onopriyenko	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	di-Ammonium Tartrate 1.1 M Sodium Acetate trihydrate .1 M	4.6
Jan 26, 2011	Olena Onopriyenko	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol 3350 25 %w/v Magnesium Chloride hexahydrate .2 M Tris .1 M	8.5
Jan 26, 2011	Olena Onopriyenko	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Sodium Chloride 3.2 M Sodium Acetate trihydrate .1 M	4.6
Jan 26, 2011	Olena Onopriyenko	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	ammonium sulfate 1.6 M Sodium Chloride .1 M HEPES .1 M	7.5
Jan 26, 2011	Olena Onopriyenko	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	tri-Ammonium Citrate pH 7.0 1 M Bis-Tris Propane .1 M	7
Jan 26, 2011	Olena Onopriyenko	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Ammonium Sulfate 2.5 M Bis-Tris Propane .1 M	7
Jan 26, 2011	Olena Onopriyenko	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Ammonium Sulfate 1.6 M Calcium Chloride dihydrate .05 M Bis-Tris	6.5
Jan 26, 2011	Olena Onopriyenko	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	tri-Sodium Citrate dihydrate .7 M Tris .1 M	8.5
Jan 26, 2011	Olena Onopriyenko	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol 4000 20 % iso-Propanole 10 % tri-Sodium Citrate dihydrate .1 M	5.6
Jan 26, 2011	Olena Onopriyenko	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	PEG2KMME 30 % Ammonium Sulfate .2 M Sodium Cacodylate .1 M	6.5
Jan 26, 2011	Olena Onopriyenko	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol 3350 25 %w/v Magnesium Chloride hexahydrate .2 M Bis-Tris .1 M	6.5
Jan 26, 2011	Olena Onopriyenko	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol 3350 20 %w/v Zinc Acetate dihydrate .05 M
Jan 26, 2011	Olena Onopriyenko	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol 3350 25 %w/v Lithium Sulfate monohydrate .2 M Bis-Tris .1 M	5.5
Jan 26, 2011	Olena Onopriyenko	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Lithium Sulfate monohydrate 1 M Bis-Tris Propane .1 M	7
Jan 26, 2011	Olena Onopriyenko	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	polyethylene glycol 400 15 %v/v Magnesium Chloride hexahydrate .2 M Sodium HEPES .1 M	7.5
Jan 26, 2011	Olena Onopriyenko	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Sodium Chloride 3.2 M Bis-Tris Propane .1 M	7
Jan 26, 2011	Olena Onopriyenko	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	polyethylene glycol 400 30 %v/v Magnesium Chloride hexahydrate .01 M Potassium Chloride .1 M Tris .05 M	8.5
Jan 26, 2011	Olena Onopriyenko	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Ammonium Sulfate 1.5 M Sodium Acetate trihydrate .1 M	4.6
Jan 26, 2011	Olena Onopriyenko	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol Monomethyl Ether 2000 30 %w/v ammonium sulfate .2 M sodium acetate trihydrate .1 M	4.6
Jan 26, 2011	Olena Onopriyenko	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol 3350 20 %w/v di-Ammonium hydrogen Citrate .2 M
Jan 26, 2011	Olena Onopriyenko	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol 3350 25 %w/v Magnesium Chloride hexahydrate .2 M HEPES .1 M	7.5
Jan 26, 2011	Olena Onopriyenko	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	polyethylene glycol 4000 30 %w/v Magnesium Chloride hexahydrate .2 M Tris .1 M	8.5
Jan 26, 2011	Olena Onopriyenko	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol 3350 20 %w/v Magnesium Formate .2 M
Jan 26, 2011	Olena Onopriyenko	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol 3350 25 %w/v Lithium Sulfate monohydrate .2 M HEPES .1 M	7.5
Jan 26, 2011	Olena Onopriyenko	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol 3350 25 %w/v Ammonium Acetate .2 M Bis-Tris .1 M	5.5
Jan 26, 2011	Olena Onopriyenko	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	polyethylene glycol 4000 30 %w/v Ammonium Acetate .2 M tri-Sodium Citrate dihydrate .1 M	5.6
Jan 26, 2011	Olena Onopriyenko	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	polyethylene glycol 4000 30 %w/v Ammonium Acetate .2 M Sodium acetate trihydrate .1 M	4.6
Jan 26, 2011	Olena Onopriyenko	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol 3350 25 % di-Sodium Tartrate dihydrate .2 M Tris .1 M	8.5
Jan 26, 2011	Olena Onopriyenko	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Jeffamine ED-2001 reagent pH 7.0 30 %v/v HEPES .1 M	7
Jan 26, 2011	Olena Onopriyenko	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol 3350 20 %w/v Sodium Sulfate decahydrate .2 M
Jan 26, 2011	Olena Onopriyenko	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	polyethylene glycol 4000 30 %w/v Sodium Acetate trihydrate .2 M Tris .1 M	8.5
Jan 26, 2011	Olena Onopriyenko	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol 3350 20 %w/v Potassium Chloride .2 M
Jan 26, 2011	Olena Onopriyenko	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol 3350 20 %w/v Potassium dihydrogen Phosphate .2 M
Jan 26, 2011	Olena Onopriyenko	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	polyethylene glycol 8000 20 %w/v Potassium dihydrogen Phosphate .05 M
Jan 26, 2011	Olena Onopriyenko	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol 3350 20 %w/v Potassium Sulfate .2 M
Jan 26, 2011	Olena Onopriyenko	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	polyethylene glycol MME 2000 1 %w/v Succinic Acid pH 7.0 1 M HEPES .1 M	7
Jan 26, 2011	Olena Onopriyenko	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol 3350 20 %w/v di-Sodium Tartrate dihydrate .2 M

Jan 26, 2011

Olena Onopriyenko

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Sodium Chloride 5 M

Jan 26, 2011

Olena Onopriyenko

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

iso-Propanol 5 %v/v ammonium sulfate 2 M

Jan 26, 2011

Olena Onopriyenko

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Ammonium Sulfate 2 M Bis-Tris .1 M

5.5

Jan 26, 2011

Olena Onopriyenko

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

iso-Propanol 5 %v/v polyethylene glycol 4000 10 %w/v Sodium HEPES .1 M

7.5

Jan 26, 2011

Olena Onopriyenko

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol 3350 20 %w/v Ammonium Formate .2 M

Jan 26, 2011

Olena Onopriyenko

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

polyethylene glycol 8000 18 %w/v Calcium Acetate hydrate .2 M Sodium Cacodylate .1 M

6.5

Jan 26, 2011

Olena Onopriyenko

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol 8000 30 %w/v Sodium Acetate trihydrate .2 M Bis-Tris

6.5

Jan 26, 2011

Olena Onopriyenko

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol 3350 20 %w/v potassium sodium tartrate tetrahydrate .2 M

Jan 26, 2011

Olena Onopriyenko

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol 3350 15 %w/v Succinic Acid pH 7.0 .1 M

Jan 26, 2011

Olena Onopriyenko

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Sodium Formate 4 M

Jan 26, 2011

Olena Onopriyenko

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol 3350 20 %w/v Sodium Thiocyanate .2 M

Jan 26, 2011

Olena Onopriyenko

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Glycerol Anhydrous 12 %v/v ammonium sulfate 1.5 M Tris .1 M

8.5

Jan 26, 2011

Olena Onopriyenko

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol 3350 20 %w/v Sodium dihydrogen Phosphate monohydrate .2 M

Jan 26, 2011

Olena Onopriyenko

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

iso-Propanol 15 %v/v Magnesium Chloride hexahydrate .025 M Potassium Chloride .1 M Sodium Cacodylate .05 M

6

Jan 26, 2011

Olena Onopriyenko

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol 3350 25 %w/v Citric Acid .1 M

3.5

Jan 26, 2011

Olena Onopriyenko

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol 3350 25 %w/v sodium acetate trihydrate .1 M

4.5

Jan 26, 2011

Olena Onopriyenko

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol 3350 25 %w/v Sodium Chloride .2 M tri-Sodium Citrate dihydrate

5.6

Jan 26, 2011

Olena Onopriyenko

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol 3350 25 %w/v Sodium Chloride .2 M Bis-Tris .1 M

5.5

Jan 26, 2011

Olena Onopriyenko

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol 3350 25 %w/v Sodium Chloride .2 M HEPES .1 M

7.5

Jan 26, 2011

Olena Onopriyenko

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol 3350 25 %w/v Tris .1 M

8.5

Jan 26, 2011

Olena Onopriyenko

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol 3350 25 %w/v Bis-Tris .1 M

6.5

Jan 26, 2011

Olena Onopriyenko

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol Monomethyl Ether 2000 30 % Tris

8.5

Jan 26, 2011

Olena Onopriyenko

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

polyethylene glycol MME 5000 20 %w/v Bis-Tris .1 M

6.5

Jan 26, 2011

Olena Onopriyenko

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol 8000 20 % Sodium Chloride .2 M Phosphate-Citrate

4.2

Jan 26, 2011

Olena Onopriyenko

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol 10,000 20 % MES .1 M

6

Jan 26, 2011

Olena Onopriyenko

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol 3350 25 %w/v ammonium sulfate .2 M Bis-Tris .1 M

5.5

Jan 26, 2011

Olena Onopriyenko

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol 3350 25 %w/v ammonium sulfate .2 M HEPES .1 M

7.5

Jan 26, 2011

Olena Onopriyenko

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

polyethylene glycol 4000 25 %w/v Ammonium Sulfate .2 M Sodium acetate trihydrate .1 M

4.6

Jan 26, 2011

Olena Onopriyenko

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

PEG 5 KMME 25 % Ammonium Sulfate .2 M tri-Sodium Citrate dihydrate

5.6

Jan 26, 2011

Olena Onopriyenko

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol 3350 25 %w/v Calcium chloride dihydrate .2 M Tris .1 M

8.5

Jan 26, 2011

Olena Onopriyenko

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol 3350 20 %w/v Calcium chloride dihydrate .2 M

Jan 26, 2011

Olena Onopriyenko

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol 3350 20 %w/v Calcium Acetate hydrate .2 M

Jan 26, 2011

Olena Onopriyenko

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

polyethylene glycol 4000 30 %w/v Lithium Sulfate monohydrate .2 M Tris .1 M

8.5

Jan 26, 2011

Olena Onopriyenko

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol 3350 20 %w/v tri-Lthium Citrate tetrahydrate .2 M

Jan 26, 2011

Olena Onopriyenko

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol 6000 10 %w/v MPD 5 %v/v HEPES .1 M

7.5

Jan 26, 2011

Olena Onopriyenko

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

ammonium sulfate 2 M potassium sodium tartrate tetrahydrate .2 M tri-Sodium Citrate dihydrate .1 M

5.6

Jan 26, 2011

Olena Onopriyenko

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Ammonium Sulfate 2.5 M Sodium Acetate trihydrate .1 M

4.6

Jan 26, 2011

Olena Onopriyenko

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Ammonium Sulfate 1.5 M Ammonium Sulfate 1.5 M Bis-Tris

6.5

Jan 26, 2011

Olena Onopriyenko

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Ammonium Sulfate 2 M PEG 2KMME 10 %

Jan 26, 2011

Olena Onopriyenko

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

polyethylene glycol 400 2 %v/v Ammonium Sulfate 2 M Sodium HEPES .1 M

7.5

Jan 26, 2011

Olena Onopriyenko

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Sodium Formate 3.5 M Sodium Acetate trihydrate .1 M

4.6

Jan 26, 2011

Olena Onopriyenko

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Sodium Nitrate 4 M Bis-Tris Propane .1 M

7

Jan 26, 2011

Olena Onopriyenko

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Sodium/Potassium Phosphate 1 M Sodium/Potassium Phosphate

6.9

Jan 26, 2011

Olena Onopriyenko

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

tri-Ammonium Citrate pH 7.0 2 M Bis-Tris Propane .1 M

7

Jan 26, 2011

Olena Onopriyenko

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

tri-Sodium Citrate dihydrate 1.4 M Sodium HEPES .1 M

7.5

Jan 26, 2011

Olena Onopriyenko

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

polyethylene glycol 400 28 %v/v Calcium chloride dihydrate .2 M Sodium HEPES .1 M

7.5

Jan 26, 2011

Olena Onopriyenko

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol Monomethyl Ether 2000 30 % Ammonium Acetate .2 M tri-Sodium Citrate dihydrate

5.6

Jan 26, 2011

Olena Onopriyenko

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

polyethylene glycol MME 5000 10 %w/v Tacsimate pH 7.0 5 %w/v HEPES .1 M

7

Jan 26, 2011

Olena Onopriyenko

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Ammonium Acetate 4 M Sodium Acetate trihydrate .1 M

4.6

Jan 26, 2011

Olena Onopriyenko

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Ammonium Chloride 1.5 M Sodium Acetate trihydrate .1 M

4.6

Jan 26, 2011

Olena Onopriyenko

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol Monomethyl Ether 5000 30 %w/v ammonium sulfate .2 M MES .1 M

6.5

Jan 26, 2011

Olena Onopriyenko

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

PEG 5KMME 25 % Ammonium Sulfate .2 M Tris .1 M

8.5

Jan 26, 2011

Olena Onopriyenko

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

polyethylene glycol 8000 30 %w/v Ammonium Sulfate .2 M Sodium Cacodylate .1 M

6.5

Jan 26, 2011

Olena Onopriyenko

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol 3350 20 %w/v Ammonium dihydrogen Phosphate .2 M

Jan 26, 2011

Olena Onopriyenko

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Ammonium dihydrogen Phosphate 2 M Tris .1 M

8.5

Jan 26, 2011

Olena Onopriyenko

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

polyethylene glycol 8000 9 %w/v Calcium Acetate hydrate .2 M Sodium Cacodylate .1 M

6.5

Jan 26, 2011

Olena Onopriyenko

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol 3350 25 %w/v Magnesium Chloride hexahydrate .2 M Sodium acetate trihydrate .1 M

4.6

Jan 26, 2011

Olena Onopriyenko

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol 3350 25 %w/v Magnesium Chloride hexahydrate .2 M tri-Sodium Citrate dihydrate .1 M

5.6

Jan 26, 2011

Olena Onopriyenko

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol 3350 25 %w/v Magnesium Chloride hexahydrate .2 M Bis-Tris .1 M

5.5

Jan 26, 2011

Olena Onopriyenko

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

di-Ammonium Tartrate 1.1 M Sodium Acetate trihydrate .1 M

4.6

Jan 26, 2011

Olena Onopriyenko

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol 3350 25 %w/v Magnesium Chloride hexahydrate .2 M Tris .1 M

8.5

Jan 26, 2011

Olena Onopriyenko

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Sodium Chloride 3.2 M Sodium Acetate trihydrate .1 M

4.6

Jan 26, 2011

Olena Onopriyenko

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

ammonium sulfate 1.6 M Sodium Chloride .1 M HEPES .1 M

7.5

Jan 26, 2011

Olena Onopriyenko

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

tri-Ammonium Citrate pH 7.0 1 M Bis-Tris Propane .1 M

7

Jan 26, 2011

Olena Onopriyenko

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Ammonium Sulfate 2.5 M Bis-Tris Propane .1 M

7

Jan 26, 2011

Olena Onopriyenko

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Ammonium Sulfate 1.6 M Calcium Chloride dihydrate .05 M Bis-Tris

6.5

Jan 26, 2011

Olena Onopriyenko

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

tri-Sodium Citrate dihydrate .7 M Tris .1 M

8.5

Jan 26, 2011

Olena Onopriyenko

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol 4000 20 % iso-Propanole 10 % tri-Sodium Citrate dihydrate .1 M

5.6

Jan 26, 2011

Olena Onopriyenko

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

PEG2KMME 30 % Ammonium Sulfate .2 M Sodium Cacodylate .1 M

6.5

Jan 26, 2011

Olena Onopriyenko

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol 3350 25 %w/v Magnesium Chloride hexahydrate .2 M Bis-Tris .1 M

6.5

Jan 26, 2011

Olena Onopriyenko

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol 3350 20 %w/v Zinc Acetate dihydrate .05 M

Jan 26, 2011

Olena Onopriyenko

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol 3350 25 %w/v Lithium Sulfate monohydrate .2 M Bis-Tris .1 M

5.5

Jan 26, 2011

Olena Onopriyenko

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Lithium Sulfate monohydrate 1 M Bis-Tris Propane .1 M

7

Jan 26, 2011

Olena Onopriyenko

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

polyethylene glycol 400 15 %v/v Magnesium Chloride hexahydrate .2 M Sodium HEPES .1 M

7.5

Jan 26, 2011

Olena Onopriyenko

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Sodium Chloride 3.2 M Bis-Tris Propane .1 M

7

Jan 26, 2011

Olena Onopriyenko

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

polyethylene glycol 400 30 %v/v Magnesium Chloride hexahydrate .01 M Potassium Chloride .1 M Tris .05 M

8.5

Jan 26, 2011

Olena Onopriyenko

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Ammonium Sulfate 1.5 M Sodium Acetate trihydrate .1 M

4.6

Jan 26, 2011

Olena Onopriyenko

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol Monomethyl Ether 2000 30 %w/v ammonium sulfate .2 M sodium acetate trihydrate .1 M

4.6

Jan 26, 2011

Olena Onopriyenko

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol 3350 20 %w/v di-Ammonium hydrogen Citrate .2 M

Jan 26, 2011

Olena Onopriyenko

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol 3350 25 %w/v Magnesium Chloride hexahydrate .2 M HEPES .1 M

7.5

Jan 26, 2011

Olena Onopriyenko

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

polyethylene glycol 4000 30 %w/v Magnesium Chloride hexahydrate .2 M Tris .1 M

8.5

Jan 26, 2011

Olena Onopriyenko

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol 3350 20 %w/v Magnesium Formate .2 M

Jan 26, 2011

Olena Onopriyenko

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol 3350 25 %w/v Lithium Sulfate monohydrate .2 M HEPES .1 M

7.5

Jan 26, 2011

Olena Onopriyenko

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol 3350 25 %w/v Ammonium Acetate .2 M Bis-Tris .1 M

5.5

Jan 26, 2011

Olena Onopriyenko

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

polyethylene glycol 4000 30 %w/v Ammonium Acetate .2 M tri-Sodium Citrate dihydrate .1 M

5.6

Jan 26, 2011

Olena Onopriyenko

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

polyethylene glycol 4000 30 %w/v Ammonium Acetate .2 M Sodium acetate trihydrate .1 M

4.6

Jan 26, 2011

Olena Onopriyenko

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol 3350 25 % di-Sodium Tartrate dihydrate .2 M Tris .1 M

8.5

Jan 26, 2011

Olena Onopriyenko

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Jeffamine ED-2001 reagent pH 7.0 30 %v/v HEPES .1 M

7

Jan 26, 2011

Olena Onopriyenko

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol 3350 20 %w/v Sodium Sulfate decahydrate .2 M

Jan 26, 2011

Olena Onopriyenko

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

polyethylene glycol 4000 30 %w/v Sodium Acetate trihydrate .2 M Tris .1 M

8.5

Jan 26, 2011

Olena Onopriyenko

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol 3350 20 %w/v Potassium Chloride .2 M

Jan 26, 2011

Olena Onopriyenko

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol 3350 20 %w/v Potassium dihydrogen Phosphate .2 M

Jan 26, 2011

Olena Onopriyenko

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

polyethylene glycol 8000 20 %w/v Potassium dihydrogen Phosphate .05 M

Jan 26, 2011

Olena Onopriyenko

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol 3350 20 %w/v Potassium Sulfate .2 M

Jan 26, 2011

Olena Onopriyenko

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

polyethylene glycol MME 2000 1 %w/v Succinic Acid pH 7.0 1 M HEPES .1 M

7

Jan 26, 2011

Olena Onopriyenko

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol 3350 20 %w/v di-Sodium Tartrate dihydrate .2 M

Purification

Related Crystallization Drops