Purification

Target
IDP97247  
Purification ID
A23260  
Expression
LB (Jun 24, 2019)  
Experiment Date Start
Jul 15, 2019  
Experiment Date End
Jul 18, 2019  
Person
Jessica Johnson  
Lab
Argonne National Laboratory  
Protocol
standard purification_ANL  
Status
success  
Description
IMAC-I, IMAC-II, SEC::ÄKTAxpress using IMAC-II program::ÄKTAxpress IMAC-II program flow-through (20 mM imidazole)  
Affinity Tag Status
cleaved  
SeMet Status
native  
Final Buffer
20 mM HEPES pH 8.0, 250 mM NaCl, 2 mM DTT  
Final Concentration (mg/mL)
6  
Final Volume (μL)
7000  
Shipped To Lab Id
ANL  
Shipped Date
Jul 17, 2019  
Inserted
Oct 24, 2019