Purification

Target
IDP97898  
Purification ID
A23284  
Expression
LB + 40 mM K2HPO4 (Oct 15, 2019)  
Experiment Date Start
Oct 23, 2019  
Experiment Date End
Oct 24, 2019  
Person
Natalia Maltseva  
Lab
Argonne National Laboratory  
Protocol
standard purification_ANL  
Status
success  
Description
IMAC-I, IMAC-II::ÄKTAxpress using IMAC-I program::ÄKTAxpress IMAC-I program eluate (250 mM imidazole)  
Affinity Tag Status
cleaved  
SeMet Status
native  
Final Buffer
20 mM HEPES pH 8.0, 250 mM NaCl, 2 mM DTT  
Final Concentration (mg/mL)
16  
Final Volume (μL)
12500  
Shipped To Lab Id
ANL  
Shipped Date
Oct 24, 2019  
Inserted
Oct 31, 2019