Purification

Target

IDP52003  

Purification ID

A23455  

Expression

LB + 40 mM K2HPO4 (Mar 26, 2020)  

Experiment Date Start

Apr 01, 2020  

Experiment Date End

Apr 03, 2020  

Person

Lucy Stols  

Lab

Argonne National Laboratory  

Protocol

standard purification_ANL  

Status

success  

Description

IMAC-I, SEC, IMAC-II::ÄKTAxpress IMAC-II program flow-through (20 mM imidazole)::concentration and buffer exchange (Amicon 3k or 10k)  

Affinity Tag Status

na  

SeMet Status

native  

Covalent Modification

 

Final Buffer

50 mM HEPES pH 8.0, 500 mM NaCl, 5 % Glycerol, 10 mM ß-mercaptoethanol, 250 mM Imidazole  

Final Concentration (mg/mL)

15  

Final Volume (μL)

4000  

Shipped To Lab Id

ANL  

Shipped Date

Apr 03, 2020  

Inserted

Jul 23, 2020