Purification

Target
IDP52003  
Purification ID
A23495  
Expression
LB + 40 mM K2HPO4 (Jun 17, 2020)  
Experiment Date Start
Jun 22, 2020  
Experiment Date End
Jun 23, 2020  
Person
Christine Tesar  
Lab
Argonne National Laboratory  
Protocol
standard purification_ANL  
Status
success  
Description
PIG-I, SEC, IMAC-II::PIG IMAC-II (PIG-II) program eluate (40 mM imidazole)::concentration and buffer exchange (Amicon 3k or 10k)  
Affinity Tag Status
cleaved  
SeMet Status
native  
Final Buffer
20 mM HEPES pH 8.0, 250 mM NaCl, 2 mM DTT  
Final Concentration (mg/mL)
41  
Final Volume (μL)
1100  
Shipped To Lab Id
ANL  
Shipped Date
Jun 24, 2020  
Inserted
Aug 17, 2020