Purification

Target

IDP98215  

Purification ID

A23584  

Expression

M9 minimal + semet (Dec 07, 2020)  

Experiment Date Start

Jan 04, 2021  

Experiment Date End

Jan 07, 2021  

Person

Christine Tesar  

Lab

Argonne National Laboratory  

Protocol

standard purification_ANL  

Status

success  

Description

PIG-I, IMAC-II::ÄKTAxpress IMAC-II program flow-through (20 mM imidazole)::concentration and buffer exchange (Amicon 3k or 10k)  

Affinity Tag Status

cleaved  

SeMet Status

semet  

Covalent Modification

 

Final Buffer

20 mM HEPES pH 8.0, 250 mM NaCl, 2 mM DTT  

Final Concentration (mg/mL)

31  

Final Volume (μL)

700  

Shipped To Lab Id

ANL  

Shipped Date

Jan 06, 2021  

Inserted

Jan 18, 2021