Purification

Target

IDP96129  

Purification ID

A23608  

Expression

M9 minimal + semet (Oct 01, 2020)  

Experiment Date Start

Oct 18, 2020  

Experiment Date End

Oct 20, 2020  

Person

Jerzy Osipiuk  

Lab

Argonne National Laboratory  

Protocol

standard purification_ANL  

Status

success  

Description

IMAC-I, IMAC-II, SEC::SEC peak 1::concentration and buffer exchange (Amicon 3k or 10k)  

Affinity Tag Status

cleaved  

SeMet Status

semet  

Covalent Modification

 

Final Buffer

50 mM HEPES pH 8.0, 500 mM NaCl, 5 % Glycerol, 10 mM ß-mercaptoethanol, 250 mM Imidazole  

Final Concentration (mg/mL)

9  

Final Volume (μL)

500  

Shipped To Lab Id

ANL  

Shipped Date

Oct 20, 2020  

Inserted

Feb 08, 2021