Purification

Target
IDP97893  
Purification ID
A23627  
Expression
LB + 40 mM K2HPO4 (Dec 21, 2019)  
Experiment Date Start
Jan 28, 2021  
Experiment Date End
Jan 29, 2021  
Person
Natalia Maltseva  
Lab
Argonne National Laboratory  
Protocol
standard purification_ANL  
Status
success  
Description
IMAC-I, IMAC-II::ÄKTAxpress using IMAC-I program::ÄKTAxpress IMAC-I program eluate (250 mM imidazole)  
Affinity Tag Status
cleaved  
SeMet Status
native  
Final Buffer
20 mM HEPES pH 8.0, 250 mM NaCl, 2 mM DTT  
Final Concentration (mg/mL)
23  
Final Volume (μL)
7500  
Shipped To Lab Id
ANL  
Shipped Date
Jan 29, 2021  
Inserted
Feb 22, 2021