Purification

Target
IDP97218  
Purification ID
A23640  
Expression
LB + 40 mM K2HPO4 (Nov 01, 2019)  
Experiment Date Start
Nov 03, 2019  
Experiment Date End
Nov 05, 2019  
Person
Mateusz Wilamowski  
Lab
Argonne National Laboratory  
Protocol
standard purification_ANL  
Status
success  
Description
IMAC-I, IMAC-II, SEC::ÄKTAxpress using IMAC-II program::ÄKTAxpress IMAC-II program flow-through (20 mM imidazole)  
Affinity Tag Status
cleaved  
SeMet Status
native  
Final Buffer
50 mM HEPES pH 8.0, 500 mM NaCl, 5 % Glycerol, 10 mM ß-mercaptoethanol, 250 mM Imidazole  
Final Concentration (mg/mL)
5  
Final Volume (μL)
20000  
Shipped To Lab Id
ANL  
Shipped Date
Nov 05, 2019  
Inserted
Mar 01, 2021