Purification

Target
IDP97908  
Purification ID
A23669  
Expression
LB (Mar 31, 2021)  
Experiment Date Start
Mar 31, 2021  
Experiment Date End
Apr 01, 2021  
Person
Natalia Maltseva  
Lab
Argonne National Laboratory  
Protocol
standard purification_ANL  
Status
success  
Description
IMAC-I, IMAC-II::ÄKTAxpress IMAC-II program flow-through (20 mM imidazole)::concentration and buffer exchange (Amicon 3k or 10k)  
Affinity Tag Status
na  
SeMet Status
native  
Final Buffer
20 mM HEPES pH 8.0, 250 mM NaCl, 2 mM DTT  
Final Concentration (mg/mL)
34  
Final Volume (μL)
500  
Shipped To Lab Id
ANL  
Shipped Date
Apr 01, 2021  
Inserted
Jun 21, 2021