Purification

Target
IDP52003  
Purification ID
A23703  
Expression
LB + 40 mM K2HPO4 (Oct 14, 2020)  
Experiment Date Start
Feb 08, 2021  
Experiment Date End
Feb 09, 2021  
Person
Christine Tesar  
Lab
Argonne National Laboratory  
Protocol
standard purification_ANL  
Status
success  
Description
IMAC-I, SEC, IMAC-II::SEC::SEC peak 1  
Affinity Tag Status
cleaved  
SeMet Status
native  
Final Buffer
20 mM HEPES pH 8.0, 250 mM NaCl, 2 mM DTT  
Final Concentration (mg/mL)
1  
Final Volume (μL)
9500  
Shipped To Lab Id
ANL  
Shipped Date
Feb 10, 2021  
Inserted
Aug 23, 2021